The interaction between Chlorpromazine (CPM) and human serum albumin (HSA) was investigated by equilibrium dialysis. Since the binding constant of CPM to HSA was independent of ionic strength and decreased with increase of alkyl chain length of fatty acid added, the interaction between CPM and HSA was considered to be due to hydrophobic mechanism. Chemical shifts in 1H-NMR spectra of CPM were independent of the concentration of CPM and addition of HSA. Spin-lattice relaxation time (T1) and spin-spin relaxation time (T2) of respective protons of CPM were independent of the concentration, but depended on the concentration of HSA added. The binding position of CPM to HSA was considered to involve the hydrophobic aromatic moiety of CPM from the ratio of spin-spin relaxation rates (1/T2) of CPM bound to HSA and free CPM.
|ホスト出版物のタイトル||Polymer Preprints, Japan - 55th SPSJ Annual Meeting|
|出版ステータス||出版済み - 2006|
|イベント||55th SPSJ Annual Meeting - Nagoya, 日本|
継続期間: 5 24 2006 → 5 26 2006
|その他||55th SPSJ Annual Meeting|
|Period||5/24/06 → 5/26/06|
All Science Journal Classification (ASJC) codes