Interaction of the hemolytic lectin, CEL-III, with cultured human leukemic cell lines

I. Sallay, S. Moriwaki, O. Nakamura, S. Yasuda, M. Kimura, N. Yamasaki, K. Itoh, H. Ohba

研究成果: Contribution to journalArticle査読

7 被引用数 (Scopus)

抄録

We studied interaction of CEL-III with cultured human leukemic cell lines and lymphocytes from normal adults by evaluating the extent of cytotoxicity and cytoagglutination. Among acute T lymphoblastic leukemia (T-ALL) cell lines, CEL-III displayed increased toxicity against different acute lymphoblastic leukemia (ALL) cell lines as a function of increasing differentiation stage. In the case of acute B lymphoblastic leukemia (B-ALL) cell lines, CEL-III showed strong cytotoxicity against relatively immature cell lines. We found that CEL-III was more toxic for ALL cell lines than leukocytes obtained from peripheral blood of healthy adults. Strong influence of the additional amount of calcium ion on the extent of cytotoxicity was observed. In addition, we describe a new way to evaluate the extent of cytoagglutination in "% of agglutinated cells." These findings make CEL-III a promising candidate in research for lectins which bind to and destroy only the targeted leukemic cells.

本文言語英語
ページ(範囲)877-883
ページ数7
ジャーナルJournal of Hematotherapy and Stem Cell Research
9
6
DOI
出版ステータス出版済み - 2000

All Science Journal Classification (ASJC) codes

  • Hematology
  • Immunology

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