Interaction of U-box-type ubiquitin-protein ligases (E3s) with molecular chaperones

Shigetsugu Hatakeyama, Masaki Matsumoto, Masayoshi Yada, Keiichi Nakayama

研究成果: ジャーナルへの寄稿記事

70 引用 (Scopus)

抜粋

Members of the U-box family of proteins constitute a class of ubiquitin-protein ligases (E3s) distinct from the HECT-type and RING finger-containing E3 families. Two representative mammalian U-box proteins, UFD2a and CHIP, interact with the molecular chaperones VCP and either Hsp90 or Hsc70, respectively, and are implicated in the degradation of damaged proteins. We have now investigated the roles of mammalian U-box proteins by performing a comprehensive screen for molecules that interact with these proteins in the yeast two-hybrid system. All mammalian U-box proteins tested were found to interact with molecular chaperones or cochaperones, including Hsp90, Hsp70, DnaJc7, EKN1, CRN, and VCP. These observations suggest that the function of U box-type E3s is to mediate the degradation of unfolded or misfolded proteins in conjunction with molecular chaperones as receptors that recognize such abnormal proteins.

元の言語英語
ページ(範囲)533-548
ページ数16
ジャーナルGenes to Cells
9
発行部数6
DOI
出版物ステータス出版済み - 6 1 2004

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All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

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