Intercellular Ca 2+ response triggered by IP 3 among endothelial cells in shear stress flow

Susumu Kudo, Makoto Hosobuchi, Shogo Kuretoko, Tateki Sumii, Tomoya Shimada, Marie Terada, Kazuo Tanishita

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)


A phenomenon has been observed in which intracellular Ca 2+ concentration in endothelial cells increases upon application of shear stress (Ca 2+ response). It is therefore assumed that Ca 2+ is the second messenger in the transfer of shear stress stimulation into cells. The Ca 2+ response is also known to spread to surrounding cells (Ca 2+ wave). We investigated the effects on Ca 2+ wave among cultured bovine aorta endothelial cells (BAECs) upon inhibiting the main intercellular signaling pathways, such as gap junction and paracrine pathways by inducing Ca 2+ wave using D-myo-inositol 1,4,5-trisphosphate, P4(5)-(l-(2-nitrophenyl)ethyl) ester trisodium salt (Caged IP 3) due to an intracellular IP 3 elevation. In addition, we investigated the Ca 2+ wave among BAECs under shear stress loading. Using Caged IP 3, local release of ATP from BAEC induced Ca 2+ wave. The Ca 2+ wave was inhibited by the inhibitors of paracrine pathways. Furthermore, the Ca 2+ response spread in the direction of the downstream under shear flow. These results suggest that paracrine pathway is dominant in both of flow and no flow conditions.

ジャーナルNihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B
出版ステータス出版済み - 2011

All Science Journal Classification (ASJC) codes

  • 凝縮系物理学
  • 機械工学


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