Interleukin-22 attenuates double-stranded RNA-induced upregulation of PD-L1 in airway epithelial cells via a STAT3-dependent mechanism

Nanae Seki, Keiko Kan-o, Koichiro Matsumoto, Satoru Fukuyama, Saaka Hamano, Ken Tonai, keiichi ota, Hiromasa Inoue, Yoichi Nakanishi

研究成果: ジャーナルへの寄稿記事

抄録

Double-stranded RNA derived from viruses induces host immune responses. PD-L1, also known as B7-H1, is an immune-checkpoint molecule associated with the escape of viruses from host immune systems, which plays a role in the persistence of viral infection, resulting in exacerbations of underlying diseases such as asthma and chronic obstructive pulmonary disease. Interleukin (IL)-22 is produced from various immune cells and has protective properties on mucosal tissue. The binding of IL-22 to IL-22 receptor induces STAT3 activation. We investigated the effect of IL-22 on the expression in airway epithelial cells in vitro and in mouse lungs in vivo after the stimulation with an analog of viral double-stranded RNA, polyinosinic-polycytidylic acid (poly I:C). Stimulation with poly I:C upregulated PD-L1 expression on BEAS-2B cells. This upregulation of PD-L1 was attenuated by IL-22 administration. STAT3 phosphorylation was induced by IL-22 and poly I:C. Treatment of cells with STAT3 siRNA abolished the effect of IL-22 on the poly I:C-induced upregulation of PD-L1. This upregulation of PD-L1 was also attenuated by IL-11, a cytokine inducing STAT3 phosphorylation, in BEAS-2B cells. In mouse lung cells in vivo, IL-22 suppressed poly I:C-induced upregulation of PD-L1. These results suggest that IL-22 attenuates virus-induced upregulation of PD-L1 in airway epithelial cells via a STAT3-dependent mechanism.

元の言語英語
ページ(範囲)242-248
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
494
発行部数1-2
DOI
出版物ステータス出版済み - 12 9 2017

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Double-Stranded RNA
Up-Regulation
Epithelial Cells
Poly C
Viruses
Poly I-C
Phosphorylation
Interleukin-11
Lung
Pulmonary diseases
interleukin-22
Immune system
RNA Viruses
Viral RNA
Virus Diseases
Interleukin-1
Chronic Obstructive Pulmonary Disease
Small Interfering RNA
Disease Progression
Immune System

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Interleukin-22 attenuates double-stranded RNA-induced upregulation of PD-L1 in airway epithelial cells via a STAT3-dependent mechanism. / Seki, Nanae; Kan-o, Keiko; Matsumoto, Koichiro; Fukuyama, Satoru; Hamano, Saaka; Tonai, Ken; ota, keiichi; Inoue, Hiromasa; Nakanishi, Yoichi.

:: Biochemical and Biophysical Research Communications, 巻 494, 番号 1-2, 09.12.2017, p. 242-248.

研究成果: ジャーナルへの寄稿記事

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