Interleukin-33 produced by M2 macrophages and other immune cells contributes to Th2 immune reaction of IgG4-related disease

Sachiko Furukawa, Masafumi Moriyama, Kensuke Miyake, Hitoshi Nakashima, Akihiko Tanaka, Takashi Maehara, Mana Iizuka-Koga, Hiroto Tsuboi, Jun Nosuke Hayashida, Noriko Ishiguro, Masaki Yamauchi, Takayuki Sumida, Seiji Nakamura

研究成果: ジャーナルへの寄稿記事

24 引用 (Scopus)

抄録

IgG4-related disease (IgG4-RD) is characterized by elevated serum IgG4 and marked infiltration of IgG4-positive cells in multiple organs. Interleukin-33 (IL-33) is a recently described cytokine that is secreted by damaged epithelial cells, macrophages, and dendritic cells, and potently activates helper T type 2 (Th2) immune responses, which have been suggested to play a major role in IgG4 production of IgG4-RD. Here, we assessed the expression of IL-33 and related molecules in the salivary glands (SGs) of patients with IgG4-RD versus that in patients with Sjögren's syndrome (SS) and controls. Expression of IL-33 and its receptor (ST2) was strongly detected around ectopic germinal centers (GCs) in the SGs from patients with IgG4-RD, whereas IL-33 was expressed only in epithelial cells in patients with SS and controls. Moreover, IL-33 and CD68+/CD163+macrophages were mainly distributed around ectopic GCs in patients with IgG4-RD. Double immunofluorescence staining showed that IL-33 expression co-localized with CD68+/CD163+macrophages. Finally, mRNA expression levels of IL-33 showed a positive correlation to those of Th2 cytokines (IL-4 and IL-13) in patients with IgG4-RD. Our data suggest that IL-33 produced by M2 macrophages might contribute to the pathogenesis of IgG4-RD via aberrant activation of Th2 immune responses.

元の言語英語
記事番号42413
ジャーナルScientific reports
7
DOI
出版物ステータス出版済み - 2 13 2017

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Immunoglobulin G
Macrophages
Germinal Center
Salivary Glands
Epithelial Cells
Interleukin-33
Cytokines
Interleukin-13
Interleukin-4
Dendritic Cells
Fluorescent Antibody Technique
Staining and Labeling
Messenger RNA
Serum

All Science Journal Classification (ASJC) codes

  • General

これを引用

Interleukin-33 produced by M2 macrophages and other immune cells contributes to Th2 immune reaction of IgG4-related disease. / Furukawa, Sachiko; Moriyama, Masafumi; Miyake, Kensuke; Nakashima, Hitoshi; Tanaka, Akihiko; Maehara, Takashi; Iizuka-Koga, Mana; Tsuboi, Hiroto; Hayashida, Jun Nosuke; Ishiguro, Noriko; Yamauchi, Masaki; Sumida, Takayuki; Nakamura, Seiji.

:: Scientific reports, 巻 7, 42413, 13.02.2017.

研究成果: ジャーナルへの寄稿記事

Furukawa, S, Moriyama, M, Miyake, K, Nakashima, H, Tanaka, A, Maehara, T, Iizuka-Koga, M, Tsuboi, H, Hayashida, JN, Ishiguro, N, Yamauchi, M, Sumida, T & Nakamura, S 2017, 'Interleukin-33 produced by M2 macrophages and other immune cells contributes to Th2 immune reaction of IgG4-related disease', Scientific reports, 巻. 7, 42413. https://doi.org/10.1038/srep42413
Furukawa, Sachiko ; Moriyama, Masafumi ; Miyake, Kensuke ; Nakashima, Hitoshi ; Tanaka, Akihiko ; Maehara, Takashi ; Iizuka-Koga, Mana ; Tsuboi, Hiroto ; Hayashida, Jun Nosuke ; Ishiguro, Noriko ; Yamauchi, Masaki ; Sumida, Takayuki ; Nakamura, Seiji. / Interleukin-33 produced by M2 macrophages and other immune cells contributes to Th2 immune reaction of IgG4-related disease. :: Scientific reports. 2017 ; 巻 7.
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abstract = "IgG4-related disease (IgG4-RD) is characterized by elevated serum IgG4 and marked infiltration of IgG4-positive cells in multiple organs. Interleukin-33 (IL-33) is a recently described cytokine that is secreted by damaged epithelial cells, macrophages, and dendritic cells, and potently activates helper T type 2 (Th2) immune responses, which have been suggested to play a major role in IgG4 production of IgG4-RD. Here, we assessed the expression of IL-33 and related molecules in the salivary glands (SGs) of patients with IgG4-RD versus that in patients with Sj{\"o}gren's syndrome (SS) and controls. Expression of IL-33 and its receptor (ST2) was strongly detected around ectopic germinal centers (GCs) in the SGs from patients with IgG4-RD, whereas IL-33 was expressed only in epithelial cells in patients with SS and controls. Moreover, IL-33 and CD68+/CD163+macrophages were mainly distributed around ectopic GCs in patients with IgG4-RD. Double immunofluorescence staining showed that IL-33 expression co-localized with CD68+/CD163+macrophages. Finally, mRNA expression levels of IL-33 showed a positive correlation to those of Th2 cytokines (IL-4 and IL-13) in patients with IgG4-RD. Our data suggest that IL-33 produced by M2 macrophages might contribute to the pathogenesis of IgG4-RD via aberrant activation of Th2 immune responses.",
author = "Sachiko Furukawa and Masafumi Moriyama and Kensuke Miyake and Hitoshi Nakashima and Akihiko Tanaka and Takashi Maehara and Mana Iizuka-Koga and Hiroto Tsuboi and Hayashida, {Jun Nosuke} and Noriko Ishiguro and Masaki Yamauchi and Takayuki Sumida and Seiji Nakamura",
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AU - Furukawa, Sachiko

AU - Moriyama, Masafumi

AU - Miyake, Kensuke

AU - Nakashima, Hitoshi

AU - Tanaka, Akihiko

AU - Maehara, Takashi

AU - Iizuka-Koga, Mana

AU - Tsuboi, Hiroto

AU - Hayashida, Jun Nosuke

AU - Ishiguro, Noriko

AU - Yamauchi, Masaki

AU - Sumida, Takayuki

AU - Nakamura, Seiji

PY - 2017/2/13

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