Interleukin‐10 selectively inhibits osteoclastogenesis by inhibiting differentiation of osteoclast progenitors into preosteoclast‐like cells in rat bone marrow culture system

Li Xin Xu, Toshio Kukita, Akiko Kukita, Takeshi Otsuka, Yoshiyuki Niho, Tadahiko Iijima

研究成果: Contribution to journalArticle査読

70 被引用数 (Scopus)

抄録

Recombinant human interleukin‐10 (hIL‐10) inhibited the formation of osteoclast‐like multinucleated cells in rat whole bone marrow cultures. The effect of hIL‐10 on the process of osteoclast formation was further examined, since the process of osteoclast formation includes the proliferation and the differentiation of osteoclast progenitors into mononuclear preosteoclasts and the fusion of preosteoclasts into multinucleated osteoclasts. In the nonadherent bone marrow cell culture system, which was free of stromal cells and formed preosteoclast‐like cells, hIL‐10 significantly inhibited the formation of preosteoclast‐like cells even at a very low concentration (0.5 U/ml). The strong inhibition appeared even after treatment with hIL‐10 for only the first 24 h of the culture. However, hIL‐10 did not affect the fusion process of preosteoclast‐like cells to form osteoclast‐like multinucleated cells in the rat coculture system of preosteoclast‐like cells with primary osteo‐blasts. Furthermore, hIL‐10 completely inhibited the colony formation induced by granulocyte macrophage colony‐stimulating factor (GM‐CSF). These findings suggest that the inhibition of osteoclastogenesis by hIL‐10 started at the early stage of the differentiation of osteoclast progenitors to preosteoclasts. © 1995 Wiley‐Liss Inc.

本文言語英語
ページ(範囲)624-629
ページ数6
ジャーナルJournal of cellular physiology
165
3
DOI
出版ステータス出版済み - 12 1995
外部発表はい

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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