Interspecific hybrids production between Lilium brownii var. colchesteri and its close relatives by ovary slice culture

Nguyen Thi Lam Hai, Michikazu Hiramatsu, Jong Hwa Kim, Jun Ichiro Masuda, Hiroshi Okubo

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

Lilium brownii var. colchesteri (BRO) has a unique trait in its flower color. The color at anthesis is yellowish cream, and changes to white after one day. To introduce this trait and establish a sexually reproductive cultivation system for cut flower production in Lilium, interspecific crosses of L. × formolongi, L. formosanum, L. longiflorum (as seed parents) and BRO (as a pollen parent) were carried out. Capsules with unviable seeds were found in all crosses. To overcome the incongruity barrier, ovary slices excised from developing ovaries at 7 to 28 days after pollination (DAP) were cultured in Murashige and Skoog medium supplemented with 40 g·L-1 sucrose, 40 g·L-1 D-mannitol and 2.5 g·L-1 gellan gum under 24 h illumination. Hybrid plantlets were obtained from crosses of L. × formolongi × BRO and L. formosanum × BRO, but not from the cross of L. longiflorum × BRO. After acclimatization, 79 (44.1%) of 179 hybrid plants produced flowers within a year of cultivation. The hybrids developed flowers changing from yellowish cream to white during anthesis as does the pollen parent. Twentyeight of 79 flowered plants sprouted two or more flower stalks, characteristics of L. formosanum. The hybrids exhibited relatively high pollen fertility and produced capsules, but no mature seed was obtained by selfpollination.

元の言語英語
ページ(範囲)191-197
ページ数7
ジャーナルJournal of the Japanese Society for Horticultural Science
81
発行部数2
DOI
出版物ステータス出版済み - 1 1 2012

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Lilium
flowers
pollen
cream
Lilium formosanum
seeds
flowering
gellan gum
Lilium longiflorum
color
cut flowers
mannitol
lighting
plantlets
acclimation
pollination
sucrose

All Science Journal Classification (ASJC) codes

  • Horticulture

これを引用

Interspecific hybrids production between Lilium brownii var. colchesteri and its close relatives by ovary slice culture. / Hai, Nguyen Thi Lam; Hiramatsu, Michikazu; Kim, Jong Hwa; Masuda, Jun Ichiro; Okubo, Hiroshi.

:: Journal of the Japanese Society for Horticultural Science, 巻 81, 番号 2, 01.01.2012, p. 191-197.

研究成果: ジャーナルへの寄稿記事

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abstract = "Lilium brownii var. colchesteri (BRO) has a unique trait in its flower color. The color at anthesis is yellowish cream, and changes to white after one day. To introduce this trait and establish a sexually reproductive cultivation system for cut flower production in Lilium, interspecific crosses of L. × formolongi, L. formosanum, L. longiflorum (as seed parents) and BRO (as a pollen parent) were carried out. Capsules with unviable seeds were found in all crosses. To overcome the incongruity barrier, ovary slices excised from developing ovaries at 7 to 28 days after pollination (DAP) were cultured in Murashige and Skoog medium supplemented with 40 g·L-1 sucrose, 40 g·L-1 D-mannitol and 2.5 g·L-1 gellan gum under 24 h illumination. Hybrid plantlets were obtained from crosses of L. × formolongi × BRO and L. formosanum × BRO, but not from the cross of L. longiflorum × BRO. After acclimatization, 79 (44.1{\%}) of 179 hybrid plants produced flowers within a year of cultivation. The hybrids developed flowers changing from yellowish cream to white during anthesis as does the pollen parent. Twentyeight of 79 flowered plants sprouted two or more flower stalks, characteristics of L. formosanum. The hybrids exhibited relatively high pollen fertility and produced capsules, but no mature seed was obtained by selfpollination.",
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