Intracellular pH changes of cultured bovine aortic endothelial cells in response to ATP addition

Takanari Kitazono, Koichiro Takeshige, Edward J. Cragoe, Shigeki Minakami

研究成果: ジャーナルへの寄稿記事

36 引用 (Scopus)

抄録

The changes of the intracellular pH (pHi) of cultured bovine aortic endothelial cells were fluorometrically monitored using 2′,7′-bis(carboxyethyl)carboxyfluorescein (BCECF). A biphasic pHi change was observed by addition of ATP: an initial acidification followed by an alkalinization of about 0.2 pH unit above the resting level of pHi 7.23. The alkalinization was dependent on [Na+]o and [H+]o, and was inhibited by 5-(N,N-hexamethylene)amiloride, indicating that the alkalinization is mediated by the Na+/H+ exchanger. The 50% effective concentration of ATP was about 1.4 μM. ADP similarly induced pHi changes, whereas AMP and adenosine were inactive. The pHi changes induced by ATP were dependent on the extracellular Ca2+, and the addition of calcium ionophore A23187 induced similar pHi changes. The results indicate that ATP activates the Na+/H+ exchanger in cultured bovine aortic endothelial cells and the activation is mediated by the P2-purinergic receptor and is dependent on the extracellular Ca2+.

元の言語英語
ページ(範囲)1304-1309
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
152
発行部数3
DOI
出版物ステータス出版済み - 5 16 1988

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Endothelial cells
Endothelial Cells
Adenosine Triphosphate
Sodium-Hydrogen Antiporter
Purinergic P2 Receptors
Calcium Ionophores
Acidification
Calcimycin
Adenosine Monophosphate
Adenosine
Adenosine Diphosphate
Chemical activation

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Intracellular pH changes of cultured bovine aortic endothelial cells in response to ATP addition. / Kitazono, Takanari; Takeshige, Koichiro; Cragoe, Edward J.; Minakami, Shigeki.

:: Biochemical and Biophysical Research Communications, 巻 152, 番号 3, 16.05.1988, p. 1304-1309.

研究成果: ジャーナルへの寄稿記事

Kitazono, Takanari ; Takeshige, Koichiro ; Cragoe, Edward J. ; Minakami, Shigeki. / Intracellular pH changes of cultured bovine aortic endothelial cells in response to ATP addition. :: Biochemical and Biophysical Research Communications. 1988 ; 巻 152, 番号 3. pp. 1304-1309.
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abstract = "The changes of the intracellular pH (pHi) of cultured bovine aortic endothelial cells were fluorometrically monitored using 2′,7′-bis(carboxyethyl)carboxyfluorescein (BCECF). A biphasic pHi change was observed by addition of ATP: an initial acidification followed by an alkalinization of about 0.2 pH unit above the resting level of pHi 7.23. The alkalinization was dependent on [Na+]o and [H+]o, and was inhibited by 5-(N,N-hexamethylene)amiloride, indicating that the alkalinization is mediated by the Na+/H+ exchanger. The 50{\%} effective concentration of ATP was about 1.4 μM. ADP similarly induced pHi changes, whereas AMP and adenosine were inactive. The pHi changes induced by ATP were dependent on the extracellular Ca2+, and the addition of calcium ionophore A23187 induced similar pHi changes. The results indicate that ATP activates the Na+/H+ exchanger in cultured bovine aortic endothelial cells and the activation is mediated by the P2-purinergic receptor and is dependent on the extracellular Ca2+.",
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AB - The changes of the intracellular pH (pHi) of cultured bovine aortic endothelial cells were fluorometrically monitored using 2′,7′-bis(carboxyethyl)carboxyfluorescein (BCECF). A biphasic pHi change was observed by addition of ATP: an initial acidification followed by an alkalinization of about 0.2 pH unit above the resting level of pHi 7.23. The alkalinization was dependent on [Na+]o and [H+]o, and was inhibited by 5-(N,N-hexamethylene)amiloride, indicating that the alkalinization is mediated by the Na+/H+ exchanger. The 50% effective concentration of ATP was about 1.4 μM. ADP similarly induced pHi changes, whereas AMP and adenosine were inactive. The pHi changes induced by ATP were dependent on the extracellular Ca2+, and the addition of calcium ionophore A23187 induced similar pHi changes. The results indicate that ATP activates the Na+/H+ exchanger in cultured bovine aortic endothelial cells and the activation is mediated by the P2-purinergic receptor and is dependent on the extracellular Ca2+.

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