Intracellular transport and processing of lysosomal cathepsin H

Yukio Nishimura, Keitaro Kato

研究成果: Contribution to journalArticle査読

28 被引用数 (Scopus)

抄録

Intracellular transport and processing of lysosomal cathepsin H was investigated in the subcellular fractions of rat liver by pulse-labeling experiments with [35S]methionine in vivo. A newly synthesized procathepsin H with a molecular weight of 41 kDa first appeared in the rough microsomal fraction at 10 min postinjection of label. This procathepsin H moved from the microsomal fraction to the Golgi subfractions at 30 min. Then a processed single-chain form with 28 kDa appeared in the lysosomal fraction at 60 min and persisted as the predominant form after 180 min. These results suggest that the propeptide-processing of procathepsin H indeed takes place in lysosomes in the course of intracellular transport from endoplasmic reticulum via Golgi complex to lysosomes.

本文言語英語
ページ(範囲)329-334
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
148
1
DOI
出版ステータス出版済み - 10 14 1987

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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