Intrathecal cytokine profile in neuropathy with anti-neurofascin 155 antibody

Hidenori Ogata, Xu Zhang, Ryo Yamasaki, Takayuki Fujii, Akira Machida, Nobutoshi Morimoto, Kenichi Kaida, Teruaki Masuda, Yukio Ando, Motoi Kuwahara, Susumu Kusunoki, Yuri Nakamura, Takuya Matsushita, Noriko Isobe, Jun ichi Kira

研究成果: ジャーナルへの寄稿記事

抄録

Objective: To characterize the CSF cytokine profile in chronic inflammatory demyelinating polyneuropathy (CIDP) patients with IgG4 anti-neurofascin 155 (NF155) antibodies (NF155+ CIDP) or those lacking anti-NF155 antibodies (NF155 CIDP). Methods: Twenty-eight CSF cytokines/chemokines/growth factors were measured by multiplexed fluorescent immunoassay in 35 patients with NF155+ CIDP, 36 with NF155 CIDP, and 28 with non-inflammatory neurological disease (NIND). Results: CSF CXCL8/IL-8, IL-13, TNF-α, CCL11/eotaxin, CCL2/MCP-1, and IFN-γ were significantly higher, while IL-1β, IL-1ra, and G-CSF were lower, in NF155+ CIDP than in NIND. Compared with NF155 CIDP, CXCL8/IL-8 and IL-13 were significantly higher, and IL-1β, IL-1ra, and IL-6 were lower, in NF155+ CIDP. CXCL8/IL-8, IL-13, CCL11/eotaxin, CXCL10/IP-10, CCL3/MIP-1α, CCL4/MIP-1β, and TNF-α levels were positively correlated with markedly elevated CSF protein, while IL-13, CCL11/eotaxin, and IL-17 levels were positively correlated with increased CSF cell counts. IL-13, CXCL8/IL-8, CCL4/MIP-1β, CCL3/MIP-1α, and CCL5/RANTES were decreased by combined immunotherapies in nine NF155+ CIDP patients examined longitudinally. By contrast, NF155 CIDP had significantly increased IFN-γ compared with NIND, and exhibited positive correlations of IFN-γ, CXCL10/IP-10, and CXCL8/IL-8 with CSF protein. Canonical discriminant analysis of cytokines/chemokines revealed that NF155+ and NF155 CIDP were separable, and that IL-4, IL-10, and IL-13 were the three most significant discriminators. Interpretation: Intrathecal upregulation of type 2 helper T (Th2) cell cytokines is characteristic of IgG4 NF155+ CIDP, while type 1 helper T cell cytokines are increased in CIDP regardless of the presence or absence of anti-NF155 antibodies, suggesting that overproduction of Th2 cell cytokines is unique to NF155+ CIDP.

元の言語英語
ページ(範囲)2304-2316
ページ数13
ジャーナルAnnals of Clinical and Translational Neurology
6
発行部数11
DOI
出版物ステータス出版済み - 11 1 2019

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Chronic Inflammatory Demyelinating Polyradiculoneuropathy
Cytokines
Antibodies
Interleukin-13
Interleukin-8
Th2 Cells
Interleukin 1 Receptor Antagonist Protein
Interleukin-1
Chemokines
Immunoglobulin G
Chemokine CCL5
Th1 Cells
Interleukin-17
Discriminant Analysis
Granulocyte Colony-Stimulating Factor
Immunoassay

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Clinical Neurology

これを引用

Intrathecal cytokine profile in neuropathy with anti-neurofascin 155 antibody. / Ogata, Hidenori; Zhang, Xu; Yamasaki, Ryo; Fujii, Takayuki; Machida, Akira; Morimoto, Nobutoshi; Kaida, Kenichi; Masuda, Teruaki; Ando, Yukio; Kuwahara, Motoi; Kusunoki, Susumu; Nakamura, Yuri; Matsushita, Takuya; Isobe, Noriko; Kira, Jun ichi.

:: Annals of Clinical and Translational Neurology, 巻 6, 番号 11, 01.11.2019, p. 2304-2316.

研究成果: ジャーナルへの寄稿記事

Ogata, H, Zhang, X, Yamasaki, R, Fujii, T, Machida, A, Morimoto, N, Kaida, K, Masuda, T, Ando, Y, Kuwahara, M, Kusunoki, S, Nakamura, Y, Matsushita, T, Isobe, N & Kira, JI 2019, 'Intrathecal cytokine profile in neuropathy with anti-neurofascin 155 antibody', Annals of Clinical and Translational Neurology, 巻. 6, 番号 11, pp. 2304-2316. https://doi.org/10.1002/acn3.50931
Ogata, Hidenori ; Zhang, Xu ; Yamasaki, Ryo ; Fujii, Takayuki ; Machida, Akira ; Morimoto, Nobutoshi ; Kaida, Kenichi ; Masuda, Teruaki ; Ando, Yukio ; Kuwahara, Motoi ; Kusunoki, Susumu ; Nakamura, Yuri ; Matsushita, Takuya ; Isobe, Noriko ; Kira, Jun ichi. / Intrathecal cytokine profile in neuropathy with anti-neurofascin 155 antibody. :: Annals of Clinical and Translational Neurology. 2019 ; 巻 6, 番号 11. pp. 2304-2316.
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title = "Intrathecal cytokine profile in neuropathy with anti-neurofascin 155 antibody",
abstract = "Objective: To characterize the CSF cytokine profile in chronic inflammatory demyelinating polyneuropathy (CIDP) patients with IgG4 anti-neurofascin 155 (NF155) antibodies (NF155+ CIDP) or those lacking anti-NF155 antibodies (NF155− CIDP). Methods: Twenty-eight CSF cytokines/chemokines/growth factors were measured by multiplexed fluorescent immunoassay in 35 patients with NF155+ CIDP, 36 with NF155− CIDP, and 28 with non-inflammatory neurological disease (NIND). Results: CSF CXCL8/IL-8, IL-13, TNF-α, CCL11/eotaxin, CCL2/MCP-1, and IFN-γ were significantly higher, while IL-1β, IL-1ra, and G-CSF were lower, in NF155+ CIDP than in NIND. Compared with NF155− CIDP, CXCL8/IL-8 and IL-13 were significantly higher, and IL-1β, IL-1ra, and IL-6 were lower, in NF155+ CIDP. CXCL8/IL-8, IL-13, CCL11/eotaxin, CXCL10/IP-10, CCL3/MIP-1α, CCL4/MIP-1β, and TNF-α levels were positively correlated with markedly elevated CSF protein, while IL-13, CCL11/eotaxin, and IL-17 levels were positively correlated with increased CSF cell counts. IL-13, CXCL8/IL-8, CCL4/MIP-1β, CCL3/MIP-1α, and CCL5/RANTES were decreased by combined immunotherapies in nine NF155+ CIDP patients examined longitudinally. By contrast, NF155− CIDP had significantly increased IFN-γ compared with NIND, and exhibited positive correlations of IFN-γ, CXCL10/IP-10, and CXCL8/IL-8 with CSF protein. Canonical discriminant analysis of cytokines/chemokines revealed that NF155+ and NF155− CIDP were separable, and that IL-4, IL-10, and IL-13 were the three most significant discriminators. Interpretation: Intrathecal upregulation of type 2 helper T (Th2) cell cytokines is characteristic of IgG4 NF155+ CIDP, while type 1 helper T cell cytokines are increased in CIDP regardless of the presence or absence of anti-NF155 antibodies, suggesting that overproduction of Th2 cell cytokines is unique to NF155+ CIDP.",
author = "Hidenori Ogata and Xu Zhang and Ryo Yamasaki and Takayuki Fujii and Akira Machida and Nobutoshi Morimoto and Kenichi Kaida and Teruaki Masuda and Yukio Ando and Motoi Kuwahara and Susumu Kusunoki and Yuri Nakamura and Takuya Matsushita and Noriko Isobe and Kira, {Jun ichi}",
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TY - JOUR

T1 - Intrathecal cytokine profile in neuropathy with anti-neurofascin 155 antibody

AU - Ogata, Hidenori

AU - Zhang, Xu

AU - Yamasaki, Ryo

AU - Fujii, Takayuki

AU - Machida, Akira

AU - Morimoto, Nobutoshi

AU - Kaida, Kenichi

AU - Masuda, Teruaki

AU - Ando, Yukio

AU - Kuwahara, Motoi

AU - Kusunoki, Susumu

AU - Nakamura, Yuri

AU - Matsushita, Takuya

AU - Isobe, Noriko

AU - Kira, Jun ichi

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Objective: To characterize the CSF cytokine profile in chronic inflammatory demyelinating polyneuropathy (CIDP) patients with IgG4 anti-neurofascin 155 (NF155) antibodies (NF155+ CIDP) or those lacking anti-NF155 antibodies (NF155− CIDP). Methods: Twenty-eight CSF cytokines/chemokines/growth factors were measured by multiplexed fluorescent immunoassay in 35 patients with NF155+ CIDP, 36 with NF155− CIDP, and 28 with non-inflammatory neurological disease (NIND). Results: CSF CXCL8/IL-8, IL-13, TNF-α, CCL11/eotaxin, CCL2/MCP-1, and IFN-γ were significantly higher, while IL-1β, IL-1ra, and G-CSF were lower, in NF155+ CIDP than in NIND. Compared with NF155− CIDP, CXCL8/IL-8 and IL-13 were significantly higher, and IL-1β, IL-1ra, and IL-6 were lower, in NF155+ CIDP. CXCL8/IL-8, IL-13, CCL11/eotaxin, CXCL10/IP-10, CCL3/MIP-1α, CCL4/MIP-1β, and TNF-α levels were positively correlated with markedly elevated CSF protein, while IL-13, CCL11/eotaxin, and IL-17 levels were positively correlated with increased CSF cell counts. IL-13, CXCL8/IL-8, CCL4/MIP-1β, CCL3/MIP-1α, and CCL5/RANTES were decreased by combined immunotherapies in nine NF155+ CIDP patients examined longitudinally. By contrast, NF155− CIDP had significantly increased IFN-γ compared with NIND, and exhibited positive correlations of IFN-γ, CXCL10/IP-10, and CXCL8/IL-8 with CSF protein. Canonical discriminant analysis of cytokines/chemokines revealed that NF155+ and NF155− CIDP were separable, and that IL-4, IL-10, and IL-13 were the three most significant discriminators. Interpretation: Intrathecal upregulation of type 2 helper T (Th2) cell cytokines is characteristic of IgG4 NF155+ CIDP, while type 1 helper T cell cytokines are increased in CIDP regardless of the presence or absence of anti-NF155 antibodies, suggesting that overproduction of Th2 cell cytokines is unique to NF155+ CIDP.

AB - Objective: To characterize the CSF cytokine profile in chronic inflammatory demyelinating polyneuropathy (CIDP) patients with IgG4 anti-neurofascin 155 (NF155) antibodies (NF155+ CIDP) or those lacking anti-NF155 antibodies (NF155− CIDP). Methods: Twenty-eight CSF cytokines/chemokines/growth factors were measured by multiplexed fluorescent immunoassay in 35 patients with NF155+ CIDP, 36 with NF155− CIDP, and 28 with non-inflammatory neurological disease (NIND). Results: CSF CXCL8/IL-8, IL-13, TNF-α, CCL11/eotaxin, CCL2/MCP-1, and IFN-γ were significantly higher, while IL-1β, IL-1ra, and G-CSF were lower, in NF155+ CIDP than in NIND. Compared with NF155− CIDP, CXCL8/IL-8 and IL-13 were significantly higher, and IL-1β, IL-1ra, and IL-6 were lower, in NF155+ CIDP. CXCL8/IL-8, IL-13, CCL11/eotaxin, CXCL10/IP-10, CCL3/MIP-1α, CCL4/MIP-1β, and TNF-α levels were positively correlated with markedly elevated CSF protein, while IL-13, CCL11/eotaxin, and IL-17 levels were positively correlated with increased CSF cell counts. IL-13, CXCL8/IL-8, CCL4/MIP-1β, CCL3/MIP-1α, and CCL5/RANTES were decreased by combined immunotherapies in nine NF155+ CIDP patients examined longitudinally. By contrast, NF155− CIDP had significantly increased IFN-γ compared with NIND, and exhibited positive correlations of IFN-γ, CXCL10/IP-10, and CXCL8/IL-8 with CSF protein. Canonical discriminant analysis of cytokines/chemokines revealed that NF155+ and NF155− CIDP were separable, and that IL-4, IL-10, and IL-13 were the three most significant discriminators. Interpretation: Intrathecal upregulation of type 2 helper T (Th2) cell cytokines is characteristic of IgG4 NF155+ CIDP, while type 1 helper T cell cytokines are increased in CIDP regardless of the presence or absence of anti-NF155 antibodies, suggesting that overproduction of Th2 cell cytokines is unique to NF155+ CIDP.

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