Intratumoral injection of dendritic cells after treatment of anticancer drugs induces tumor-specific antitumor effect in vivo

Fumiaki Tanaka, Hiroshi Yamaguchi, Mitsuhiko Ohta, Kohjiro Mashino, Hideto Sonoda, Noriaki Sadanaga, Hiroshi Inoue, Masaki Mori

研究成果: ジャーナルへの寄稿学術誌査読

57 被引用数 (Scopus)

抄録

We investigated the in vivo antitumor effects of intratumoral (i.t.) administration of dendritic cells (DC) after low-dose chemotherapy using cisplatin + 5-FU. Combination of i.t. injection of DC and systemic chemotherapy induced complete rejection of the treated tumor, MC38 murine adenocarcinoma. Furthermore, the antitumor effects were also observed on a distant tumor inoculated in the contralateral flank of the animal. When 10x the number of tumor cells were inoculated, the antitumor effect of the combination of DC after chemotherapy was also confirmed and in comparison to that of DC or chemotherapy alone, thereafter contributed to a greater prolongation of survival. To analyze the mechanisms of the systemic antitumor effect generated in this system, we assessed the cytolytic activity against inoculated tumors. The cytolytic activity of effector cells from treated animals was shown to be tumor-specific and was mainly CD8 and MHC Class-I (p < 0.0 1) restricted. CD4 and MHC Class-II treatment marginally inhibited the cytolytic activity but not significantly (p = 0.07, 0.08 respectively). The cytolysis of effector cells was enhanced more significantly by the treatment of both DC and chemotherapy, than that of either DC or chemotherapy alone. Our study suggests that the strategy of i.t. injection of DC after low-dose chemotherapy could be a powerful weapon to treat patients with cancer in the clinical settings.

本文言語英語
ページ(範囲)265-269
ページ数5
ジャーナルInternational Journal of Cancer
101
3
DOI
出版ステータス出版済み - 9月 20 2002

!!!All Science Journal Classification (ASJC) codes

  • 腫瘍学
  • 癌研究

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