TY - JOUR
T1 - Introgressive hybridization in two indo-west pacific rhizophora mangrove species, R. mucronata and R. stylosa
AU - Ng, Wei Lun
AU - Szmidt, Alfred E.
N1 - Funding Information:
We are grateful to Dr. Hung Tuck Chan, Dr. Iskandar Z. Siregar, and Prof. Cecep Kusmana for assisting with sample collection in Malaysia and Indonesia. Dr. Hung Tuck Chan and anonymous reviewers provided helpful comments and suggestions to an earlier draft of the manuscript. This study was supported in part by the Global Environment Research Fund (D-0901) from the Ministry of Environment, Japan , and the Global COE Program, Ministry of Education, Culture, Sports, Science & Technology, Japan .
Publisher Copyright:
© 2014 Elsevier B.V.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Natural hybridization is common in plants, including mangroves. Three Rhizophora mangrove species are recognized in the Indo-West Pacific region, namely R. apiculata, R. mucronata, and R. stylosa. So far, R. apiculata has been known to form sterile hybrid offspring with R. mucronata (= R. x annamalayana) and with R. stylosa (= R. x lamarckii). A third hybrid between morphologically similar R. mucronata and R. stylosa was only recently shown to exist, via DNA sequencing. However, it has been suspected that this newly discovered hybrid may be fertile, with potential to interbreed to give rise to advanced-generation hybrids in locations where both parental species occur. In this study, inter-simple sequence repeat (ISSR) markers were used to survey several stands where R. mucronata and R. stylosa co-occur. By screening through 26 ISSR primers, a set of species-specific diagnostic bands for each species were first identified from standard samples before being used to genotype the test samples. A chloroplast DNA locus was also sequenced in the test samples to determine the direction of hybridization. Results showed that hybridization occurred between R. mucronata and R. stylosa in all investigated locations with differing levels of introgression, and that it could happen in any direction.
AB - Natural hybridization is common in plants, including mangroves. Three Rhizophora mangrove species are recognized in the Indo-West Pacific region, namely R. apiculata, R. mucronata, and R. stylosa. So far, R. apiculata has been known to form sterile hybrid offspring with R. mucronata (= R. x annamalayana) and with R. stylosa (= R. x lamarckii). A third hybrid between morphologically similar R. mucronata and R. stylosa was only recently shown to exist, via DNA sequencing. However, it has been suspected that this newly discovered hybrid may be fertile, with potential to interbreed to give rise to advanced-generation hybrids in locations where both parental species occur. In this study, inter-simple sequence repeat (ISSR) markers were used to survey several stands where R. mucronata and R. stylosa co-occur. By screening through 26 ISSR primers, a set of species-specific diagnostic bands for each species were first identified from standard samples before being used to genotype the test samples. A chloroplast DNA locus was also sequenced in the test samples to determine the direction of hybridization. Results showed that hybridization occurred between R. mucronata and R. stylosa in all investigated locations with differing levels of introgression, and that it could happen in any direction.
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U2 - 10.1016/j.aquabot.2014.07.006
DO - 10.1016/j.aquabot.2014.07.006
M3 - Article
AN - SCOPUS:85027938100
VL - 120
SP - 222
EP - 228
JO - Aquatic Botany
JF - Aquatic Botany
SN - 0304-3770
IS - PB
ER -