Intronic regulation of Aire expression by Jmjd6 for self-tolerance induction in the thymus

Toyoshi Yanagihara, Fumiyuki Sanematsu, Tetsuya Sato, Takehito Uruno, Xuefeng Duan, Takahiro Tomino, Yosuke Harada, Mayuki Watanabe, Yuqing Wang, Yoshihiko Tanaka, Yoichi Nakanishi, Mikita Suyama, Fukui Yoshinori

研究成果: ジャーナルへの寄稿学術誌査読

24 被引用数 (Scopus)

抄録

The thymus has spatially distinct microenvironments, the cortex and the medulla, where the developing T-cells are selected to mature or die through the interaction with thymic stromal cells. To establish the immunological self in the thymus, medullary thymic epithelial cells (mTECs) express diverse sets of tissue-specific self-antigens (TSAs). This ectopic expression of TSAs largely depends on the transcriptional regulator Aire, yet the mechanism controlling Aire expression itself remains unknown. Here, we show that Jmjd6, a dioxygenase that catalyses lysyl hydroxylation of splicing regulatory proteins, is critical for Aire expression. Although Jmjd6 deficiency does not affect abundance of Aire transcript, the intron 2 of Aire gene is not effectively spliced out in the absence of Jmjd6, resulting in marked reduction of mature Aire protein in mTECs and spontaneous development of multi-organ autoimmunity in mice. These results highlight the importance of intronic regulation in controlling Aire protein expression.

本文言語英語
論文番号8820
ジャーナルNature communications
6
DOI
出版ステータス出版済み - 11月 4 2015

!!!All Science Journal Classification (ASJC) codes

  • 化学 (全般)
  • 生化学、遺伝学、分子生物学(全般)
  • 物理学および天文学(全般)

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