Investigation of the active site of an unclassified glutathione transferase in Bombyx mori by alanine scanning

Kohji Yamamoto, Misuzu Yamaguchi, Naotaka Yamada

研究成果: Contribution to journalArticle査読

抄録

Glutathione transferase (GST) is an important class of detoxification enzymes that are vital for defense against various xenobiotics and cellular oxidative stress. Previously, we had reported an unclassified glutathione transferase 2 in Bombyx mori (bmGSTu2) to be responsible for detoxifying diazinon. In this study, we aimed to identify the amino acid residues that constitute a hydrogen-bonding network important for GST activity. Site-directed mutagenesis of bmGSTu2 suggested that residues Asn102, Pro162, and Ser166 contribute to its catalytic activity.

本文言語英語
ページ(範囲)238-240
ページ数3
ジャーナルJournal of Pesticide Science
45
4
DOI
出版ステータス出版済み - 2020

All Science Journal Classification (ASJC) codes

  • 昆虫科学
  • 健康、毒物学および変異誘発

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