Investigation of the proteins adsorbed on surfaces of resin applied to the extracorporeal granulocyte depletion system

K. Miyamoto, T. Nakamura, Masayuki Tokita, T. Komai, M. Yonekawa, A. Kawamura, S. Adachi

研究成果: ジャーナルへの寄稿記事

抄録

Based on the concept that the granulocyte ratio (G/L) in cancer patients suggested the increase of G/L closely related to the tumor growth and the decrease to the depletion, we have been investigating the extracorporeal granulocyte adsorption system, depleting granulocyte mechanically, for the treatment of tumor bearing hosts. In order to clarify the therapeutic efficiency of this system, we have investigated the proteins adsorbed on the surfaces of granulocyte adsorption resin which was clinically applied. Extracted proteins obtained from the resin was analyzed with gel electrophoresis (SDS-Page) and ion exchange chromatography. These analyzed results revealed that the characteristic protein was only extracted with saline containing heparin, molecular weight of the protein was estimated over 300 kD. In Chromatogram, the peak of extracted proteins existed at the same position as that of Cellular Fibronectin-Heparin complex (cFN-Hep).

元の言語英語
ページ(範囲)690-694
ページ数5
ジャーナルJapanese Journal of Artificial Organs
23
発行部数3
出版物ステータス出版済み - 1 1 1994

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Granulocytes
Proteins
Adsorption
Heparin
Neoplasms
Ion Exchange Chromatography
Fibronectins
Electrophoresis
Molecular Weight
Gels
Therapeutics
Growth

All Science Journal Classification (ASJC) codes

  • Biophysics

これを引用

Miyamoto, K., Nakamura, T., Tokita, M., Komai, T., Yonekawa, M., Kawamura, A., & Adachi, S. (1994). Investigation of the proteins adsorbed on surfaces of resin applied to the extracorporeal granulocyte depletion system. Japanese Journal of Artificial Organs, 23(3), 690-694.

Investigation of the proteins adsorbed on surfaces of resin applied to the extracorporeal granulocyte depletion system. / Miyamoto, K.; Nakamura, T.; Tokita, Masayuki; Komai, T.; Yonekawa, M.; Kawamura, A.; Adachi, S.

:: Japanese Journal of Artificial Organs, 巻 23, 番号 3, 01.01.1994, p. 690-694.

研究成果: ジャーナルへの寄稿記事

Miyamoto, K, Nakamura, T, Tokita, M, Komai, T, Yonekawa, M, Kawamura, A & Adachi, S 1994, 'Investigation of the proteins adsorbed on surfaces of resin applied to the extracorporeal granulocyte depletion system', Japanese Journal of Artificial Organs, 巻. 23, 番号 3, pp. 690-694.
Miyamoto K, Nakamura T, Tokita M, Komai T, Yonekawa M, Kawamura A その他. Investigation of the proteins adsorbed on surfaces of resin applied to the extracorporeal granulocyte depletion system. Japanese Journal of Artificial Organs. 1994 1 1;23(3):690-694.
Miyamoto, K. ; Nakamura, T. ; Tokita, Masayuki ; Komai, T. ; Yonekawa, M. ; Kawamura, A. ; Adachi, S. / Investigation of the proteins adsorbed on surfaces of resin applied to the extracorporeal granulocyte depletion system. :: Japanese Journal of Artificial Organs. 1994 ; 巻 23, 番号 3. pp. 690-694.
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abstract = "Based on the concept that the granulocyte ratio (G/L) in cancer patients suggested the increase of G/L closely related to the tumor growth and the decrease to the depletion, we have been investigating the extracorporeal granulocyte adsorption system, depleting granulocyte mechanically, for the treatment of tumor bearing hosts. In order to clarify the therapeutic efficiency of this system, we have investigated the proteins adsorbed on the surfaces of granulocyte adsorption resin which was clinically applied. Extracted proteins obtained from the resin was analyzed with gel electrophoresis (SDS-Page) and ion exchange chromatography. These analyzed results revealed that the characteristic protein was only extracted with saline containing heparin, molecular weight of the protein was estimated over 300 kD. In Chromatogram, the peak of extracted proteins existed at the same position as that of Cellular Fibronectin-Heparin complex (cFN-Hep).",
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AU - Kawamura, A.

AU - Adachi, S.

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