Involvement of cAMP signaling in elicitor-induced phytoalexin accumulation in Cupressus lusitanica cell cultures

Jian Zhao, Yingqing Guo, Koki Fujita, Kokki Sakai

研究成果: ジャーナルへの寄稿記事

30 引用 (Scopus)

抄録

• An increasing body of evidence on plant electrophysiology, biochemistry, and molecular biology shows that cAMP exists in higher plants and plays a role in several physiological processes by affecting potassium (K +) or calcium (Ca2+) fluxes. Our study here reports that cAMP is involved in elicitor-induced accumulation of a phytoalexin, β-thujaplicin, in Cupressus lusitanica cell cultures. • Treatment of C. lusitanica cultured cells with cAMP or its analogues stimulated β-thujaplicin accumulation. Cholera toxin and forskolin, activators of adenylyl cyclase, also stimulated β-thujaplicin accumulation. Enzyme immunoassay showed that after elicitor treatment, cAMP level in the elicited cells quickly increased to about three-to five-fold over the control. Cholera toxin and forskolin also stimulated cAMP accumulation in the absence of elicitor. • However, K+ and Ca2+ channel blockers inhibited the β-thujaplicin accumulation induced by cAMP analogues, suggesting that the cAMP-stimulated β-thujaplicin accumulation may involve Ca2+ and K+ fluxes. Several ionophores mimicked cAMP induction of β-thujaplicin accumulation. • Cross-talk between cAMP treatment and the ethylene signaling pathway was also observed to work in the cell cultures via Ca2+ signaling. The study also indicates an involvement of protein kinase cascades in cAMP signaling processes, leading to both phytoalexin and ethylene production.

元の言語英語
ページ(範囲)723-733
ページ数11
ジャーナルNew Phytologist
161
発行部数3
DOI
出版物ステータス出版済み - 3 1 2004

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Cupressus lusitanica
Cupressus
phytoalexins
cell culture
Cell Culture Techniques
Cholera Toxin
Colforsin
calcium
forskolin
cholera toxin
Physiological Phenomena
Electrophysiology
Ionophores
Immunoenzyme Techniques
Adenylyl Cyclases
Biochemistry
Protein Kinases
Molecular Biology
Cultured Cells
Potassium

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

これを引用

Involvement of cAMP signaling in elicitor-induced phytoalexin accumulation in Cupressus lusitanica cell cultures. / Zhao, Jian; Guo, Yingqing; Fujita, Koki; Sakai, Kokki.

:: New Phytologist, 巻 161, 番号 3, 01.03.2004, p. 723-733.

研究成果: ジャーナルへの寄稿記事

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