Involvement of G i/o in the PAR-4-induced NO production in endothelial cells

Fumi Momota, Katsuya Hirano, Mayumi Hirano, Junji Nishimura, Hideo Kanaide

研究成果: ジャーナルへの寄稿記事

12 引用 (Scopus)

抄録

We investigated the involvement of G i/o protein in NO production following the activation of proteinase-activated receptor-4 (PAR-4) in cultured bovine aortic endothelial cells. AYPGKF-NH 2 (PAR-4 activating peptide), thrombin, and ionomycin induced a concentration-dependent NO production, with the maximal production seen at 30 μM, 0.1 U/ml, and 1 μM, respectively. Ionomycin elevated [Ca 2+ ] i in a concentration-dependent manner. However, AYPGKF-NH 2 and thrombin induced no [Ca 2+ ] i elevation. The loading of cells with BAPTA almost completely inhibited both the NO production and [Ca 2+ ] i elevation induced by 1 μM ionomycin, while it had no significant effect on the AYPGKF-NH 2 -induced NO production. Treatment with pertussis toxin inhibited the AYPGKF-NH 2 -induced NO production, while it had no effect on the ionomycin-induced NO production. Our findings thus demonstrate, for the first time, that PAR-4 activation induced NO production in a manner mostly independent of the Ca 2+ signal and also that G i/o is involved in such NO production in vascular endothelial cells.

元の言語英語
ページ(範囲)365-371
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
342
発行部数2
DOI
出版物ステータス出版済み - 4 7 2006

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Proteinase-Activated Receptors
Ionomycin
Endothelial cells
Endothelial Cells
thrombin receptor peptide SFLLRNP
Gi-Go GTP-Binding Protein alpha Subunits
Pertussis Toxin
Thrombin
Peptide Hydrolases
Chemical activation
PAR-2 Receptor
alanyl-tyrosyl-prolyl-glycyl-lysyl-phenylalanine

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Involvement of G i/o in the PAR-4-induced NO production in endothelial cells . / Momota, Fumi; Hirano, Katsuya; Hirano, Mayumi; Nishimura, Junji; Kanaide, Hideo.

:: Biochemical and Biophysical Research Communications, 巻 342, 番号 2, 07.04.2006, p. 365-371.

研究成果: ジャーナルへの寄稿記事

Momota, Fumi ; Hirano, Katsuya ; Hirano, Mayumi ; Nishimura, Junji ; Kanaide, Hideo. / Involvement of G i/o in the PAR-4-induced NO production in endothelial cells :: Biochemical and Biophysical Research Communications. 2006 ; 巻 342, 番号 2. pp. 365-371.
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