Involvement of p27(KIP1) in proliferation of the retinal pigment epithelium and ciliary body

Kazuhiko Yoshida, Keiko Nakayama, Satoru Kase, Hiroyasu Nagahama, Takayuki Harada, Hiromi Ikeda, Chikako Harada, Junko Imaki, Kazuhiro Ohgami, Kenji Shiratori, Shigeaki Ohno, Shinzo Nishi, Keiichi Nakayama

研究成果: ジャーナルへの寄稿記事

15 引用 (Scopus)

抄録

According to observations in various cell lines, elimination of the cyclin-dependent kinase inhibitor p27(KIP1) during the late G1 phase of the cell cycle is required for progression to the S phase. Eyes from C57BL/6 mice at embryonic days 13, 14, and 18, and at 4 weeks of age, were analyzed by a bromodeoxyuridine cell proliferation assay and by immunocytochemistry using anti-p27(KIP1) antibody. On embryonic days 14 and 18, p27(KIP1) was detected in the ciliary body. This protein also was detected in the nuclei of the many cells of the retinal pigment epithelium on embryonic day 18, and was present in all such cells at 4 weeks of age. When p27(KIP1)-/- knockout and control mice were injected with bromodeoxyuridine between postnatal days 7 and 10 and analyzed on day 11, positive cells were abundant in the retinal pigment epithelium and the ciliary body of p27(KIP1)-/- mice, whereas few cells were positive in control mice. By fluorescent nuclear staining in whole mounts of retinal pigment epithelium at 12 weeks of age, more nuclei were present in p27(KIP1)-/- than in the wild-type mice. These results suggest that p27(KIP1) was involved in regulation of proliferation in the RPE and the ciliary body.

元の言語英語
ページ(範囲)145-150
ページ数6
ジャーナルAnatomy and Embryology
208
発行部数2
DOI
出版物ステータス出版済み - 5 1 2004

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Ciliary Body
Retinal Pigment Epithelium
Bromodeoxyuridine
Cyclin-Dependent Kinase Inhibitor p27
G1 Phase
Cell Nucleus
S Phase
Inbred C57BL Mouse
Knockout Mice
Cell Cycle
Immunohistochemistry
Cell Proliferation
Staining and Labeling
Cell Line
Antibodies
Proteins

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Embryology
  • Developmental Biology
  • Cell Biology

これを引用

Involvement of p27(KIP1) in proliferation of the retinal pigment epithelium and ciliary body. / Yoshida, Kazuhiko; Nakayama, Keiko; Kase, Satoru; Nagahama, Hiroyasu; Harada, Takayuki; Ikeda, Hiromi; Harada, Chikako; Imaki, Junko; Ohgami, Kazuhiro; Shiratori, Kenji; Ohno, Shigeaki; Nishi, Shinzo; Nakayama, Keiichi.

:: Anatomy and Embryology, 巻 208, 番号 2, 01.05.2004, p. 145-150.

研究成果: ジャーナルへの寄稿記事

Yoshida, K, Nakayama, K, Kase, S, Nagahama, H, Harada, T, Ikeda, H, Harada, C, Imaki, J, Ohgami, K, Shiratori, K, Ohno, S, Nishi, S & Nakayama, K 2004, 'Involvement of p27(KIP1) in proliferation of the retinal pigment epithelium and ciliary body', Anatomy and Embryology, 巻. 208, 番号 2, pp. 145-150. https://doi.org/10.1007/s00429-004-0382-5
Yoshida, Kazuhiko ; Nakayama, Keiko ; Kase, Satoru ; Nagahama, Hiroyasu ; Harada, Takayuki ; Ikeda, Hiromi ; Harada, Chikako ; Imaki, Junko ; Ohgami, Kazuhiro ; Shiratori, Kenji ; Ohno, Shigeaki ; Nishi, Shinzo ; Nakayama, Keiichi. / Involvement of p27(KIP1) in proliferation of the retinal pigment epithelium and ciliary body. :: Anatomy and Embryology. 2004 ; 巻 208, 番号 2. pp. 145-150.
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abstract = "According to observations in various cell lines, elimination of the cyclin-dependent kinase inhibitor p27(KIP1) during the late G1 phase of the cell cycle is required for progression to the S phase. Eyes from C57BL/6 mice at embryonic days 13, 14, and 18, and at 4 weeks of age, were analyzed by a bromodeoxyuridine cell proliferation assay and by immunocytochemistry using anti-p27(KIP1) antibody. On embryonic days 14 and 18, p27(KIP1) was detected in the ciliary body. This protein also was detected in the nuclei of the many cells of the retinal pigment epithelium on embryonic day 18, and was present in all such cells at 4 weeks of age. When p27(KIP1)-/- knockout and control mice were injected with bromodeoxyuridine between postnatal days 7 and 10 and analyzed on day 11, positive cells were abundant in the retinal pigment epithelium and the ciliary body of p27(KIP1)-/- mice, whereas few cells were positive in control mice. By fluorescent nuclear staining in whole mounts of retinal pigment epithelium at 12 weeks of age, more nuclei were present in p27(KIP1)-/- than in the wild-type mice. These results suggest that p27(KIP1) was involved in regulation of proliferation in the RPE and the ciliary body.",
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AU - Kase, Satoru

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AU - Harada, Takayuki

AU - Ikeda, Hiromi

AU - Harada, Chikako

AU - Imaki, Junko

AU - Ohgami, Kazuhiro

AU - Shiratori, Kenji

AU - Ohno, Shigeaki

AU - Nishi, Shinzo

AU - Nakayama, Keiichi

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