Involvement of p27KIP1 degradation by SKp2 in the regulation of proliferation in response to wounding of corneal epithelium

Kazuhiko Yoshida, Keiko Nakayama, Hiroyasu Nagahama, Takayuki Harada, Chikako Harada, Junko Imaki, Akira Matsuda, Kazuyuki Yamamoto, Miyuki Ito, Shigeaki Ohno, Keiichi Nakayama

研究成果: Contribution to journalArticle査読

27 被引用数 (Scopus)

抄録

PURPOSE. To examine the expression of the p27KIP1 in the normal and epithelial-scraped cornea and whether degradation of p27KIP1 by Skp2 is involved in the regulation of cell proliferation in response to wounding of the corneal epithelium. METHODS. C57B16, p27KIP1-/-, Skp2-/-, and Skp2-/-/p27KIP1-/- double-knockout mice were examined. Normal and epithelial-scraped corneas were analyzed by immunocytochemistry using anti-p27KIP1 antibody. Cells in the S phase of DNA synthesis were analyzed by immunocytochemistry using anti-bromodeoxyuridine (BrdU) antibody. RESULTS. The p27KIP1 was expressed in basal cells of the central and peripheral region of the cornea and limbus. This expression was not detected 24 hours after the epithelial scraping, when there were many cells in the S phase of DNA synthesis in the corneal epithelium. There were no obvious differences in the thickness and anti-BrdU staining in the corneal epithelium of p27KIP1-/- mice from that of control animals. Twenty-four hours after epithelial scraping in the Skp2-/- mice, the corneal epithelium was thinner than in wild-type mice and had many p27KIP1-positive cells and few BrdU-positive cells. In contrast, 24 hours after epithelial scraping in the Skp2-/- /p27KIP1-/- double-knockout mice, the corneal epithelium was as thick as in wild-type mice and had many BrdU-positive cells. CONCLUSIONS. These results suggest that degradation of p27KIP1 by Skp2 is involved in the regulation of cell proliferation in response to wounding of the corneal epithelium.

本文言語英語
ページ(範囲)364-370
ページ数7
ジャーナルInvestigative Ophthalmology and Visual Science
43
2
出版ステータス出版済み - 2 11 2002
外部発表はい

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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