Involvement of protein kinase in environmental stress-induced activation of human multidrug resistance 1 (MDR1) gene promoter

Takeshi Uchiumi, Kimitoshi Kohno, Hideyuki Tanimura, Katsuhiko Hidaka, Keizo Asakuno, Hiroko Abe, Yuzo Uchida, Michihiko Kuwano

研究成果: Contribution to journalArticle査読

53 被引用数 (Scopus)

抄録

The human MDR1 gene can be induced in response to various environmental stimuli. To examine whether such stress-induced activation of the MDR1 gene can be modulated by protein kinase, we employed a stable human cancer KB cell line which contained the bacterial chloramphenicol acetyltransferase (CAT) gene directed by the MDR1 gene promoter. H-7, a protein kinase C inhibitor, at more than 40 μM inhibited activation of the MDR1 promoter that was induced by ethylmethane sulfonate, 5-fluorouracil or UV irradiation. DNA binding activity of nuclear factors recognizing the MDR1 promoter was augmented in KB cells treated with UV, but decreased in cells treated concomitantly with H-7. Okadaic acid alone was able to induce the promoter activation, and this induction was dependent on specific promoter sequences. Okadaic acid also enhanced the DNA binding activity of nuclear factors recognizing the MDR1 promoter. The phosphorylation of transacting factors may modulate the MDR1 gene promoter activity.

本文言語英語
ページ(範囲)11-16
ページ数6
ジャーナルFEBS Letters
326
1-3
DOI
出版ステータス出版済み - 7 1993
外部発表はい

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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