Involvement of suppressor of cytokine signaling-1 in globular adiponectin-induced granulocyte colony-stimulating factor in RAW 264 cell

Akie Fujimoto, Sumio Akifusa, Takao Hirofuji, Yoshihisa Yamashita

研究成果: Contribution to journalArticle査読

3 被引用数 (Scopus)

抄録

We previously demonstrated that treatment with a globular type of adiponectin (gAd) induced expression of granulocyte colony-stimulating factor (G-CSF) via the MEK/ERK signaling pathway in a murine macrophage cell line, RAW 264. In the present study, we investigated whether suppressor of cytokine signaling-1 (SOCS1) has roles in the regulation of gAd-induced G-CSF generation. Intracellular G-CSF generation induced by gAd treatment peaked after 10. h and then attenuated. SOCS1 mRNA and protein were expressed at 1. h and 4. h after gAd treatment, respectively. Overexpression of SOCS1 reduced G-CSF generation and phosphorylation of ERK, JNK, and p38 MAPK in gAd-treated cells. While gAd treatment induced the translocation of STAT3 to the nucleus under control conditions, STAT3 stayed in the cytosol when SOCS1 was overexpressed. Additionally, knockdown of SOCS1 by interfering RNA caused levels of G-CSF to continue to rise beyond 10. h after gAd treatment. These results suggest that SOCS1 is involved in providing negative feedback for gAd-induced production of G-CSF.

本文言語英語
ページ(範囲)2052-2058
ページ数7
ジャーナルMolecular Immunology
48
15-16
DOI
出版ステータス出版済み - 9 1 2011

All Science Journal Classification (ASJC) codes

  • Immunology
  • Molecular Biology

フィンガープリント 「Involvement of suppressor of cytokine signaling-1 in globular adiponectin-induced granulocyte colony-stimulating factor in RAW 264 cell」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル