Involvement of the ryanodine-sensitive Ca2+ store in GLP-1-induced Ca2+ oscillations in insulin-secreting HIT cells

Sadao Sasaki, Ikuko Nakagaki, Hisao Kondo, Seiki Hori

研究成果: Contribution to journalArticle査読

13 被引用数 (Scopus)

抄録

We investigated intracellular Ca2+ ([Ca2+]i) oscillations evoked by glucagon-like peptide 1 (GLP-1) in relation to the ryanodine receptor (RyR) and Ca2+-induced Ca2+ release (CICR) mechanism in pancreatic B cell HIT. GLP-1 produced [Ca2+]i oscillations in the cells, both in media with and without Ca2+, an effect inhibited by ruthenium red and mimicked by 8-Br-cAMPS. In addition, the GLP-1-evoked [Ca2+]i rise was initiated at the local intercellular peripheral cytoplasm, and a resultant expansion of the intercellular space was also observed. Caffeine induced [Ca2+]i elevation in the medium with or without Ca2+, an effect inhibited by ruthenium red. GLP-1-evoked [Ca2+]i oscillations were also enhanced by IBMX, and eliminated by Rp-8-Br-cAMPS or 20 μM H-89 treatments whereas they were unaffected by 2 μM H-89 treatment. Forskolin caused a transient elevation in [Ca2+]i that was reduced by Rp-8-Br-cAMPS, 2 μM or 20 μM H-89. Our results indicate that GLP-1 initially generated a local [Ca2+]i elevation at the peripheral cytoplasm, subsequently producing [Ca2+]i oscillations that were inhibited by ruthenium red, involving ryanodine-sensitive and cAMP-activated CICR mechanisms. The cytoplasmic levels of cAMP as well as local Ca2+ might be responsible for [Ca2+]i oscillations.

本文言語英語
ページ(範囲)342-351
ページ数10
ジャーナルPflugers Archiv European Journal of Physiology
445
3
DOI
出版ステータス出版済み - 1 1 2002
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生理学
  • 臨床生化学
  • 生理学(医学)

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