TY - JOUR
T1 - Ionic properties of IK,n in outer hair cells of guinea pig cochlea
AU - Nakagawa, Takashi
AU - Kakehata, Seiji
AU - Yamamoto, Takehito
AU - Akaike, Norio
AU - Komune, Shizuo
AU - Uemura, Takuya
PY - 1994/10/24
Y1 - 1994/10/24
N2 - The ionic properties of voltage-dependent K+ current activated at the resting membrane potential (IK,n) of outer hair cells (OHC) isolated from the guinea pig cochlea were studied using a patch-clamp technique in a whole-cell recording mode. The reversal potential of IK,n indicated a high selectivity for K+, and the relative permeability ratios for various monovalent cations were K+ : Rb+ : NH+4 = 1 : 1.21 : 0.13. Decrease in extracellular Cl-1 inhibited the IK,n. IK,n was blocked by Cs+ and Ba2+, although the inhibitory manner of Cs+ and Ba2+ were voltage-dependent and voltage-independent, respectively. By the use of puff-application method, the local application of Ba2+ to basolateral surface of OHC shifted the holding current level in an inward direction, whereas the application to apex and hair showed little change. Indicating that the IK,n channels preferentially locate at the basolateral region of cell membrane.
AB - The ionic properties of voltage-dependent K+ current activated at the resting membrane potential (IK,n) of outer hair cells (OHC) isolated from the guinea pig cochlea were studied using a patch-clamp technique in a whole-cell recording mode. The reversal potential of IK,n indicated a high selectivity for K+, and the relative permeability ratios for various monovalent cations were K+ : Rb+ : NH+4 = 1 : 1.21 : 0.13. Decrease in extracellular Cl-1 inhibited the IK,n. IK,n was blocked by Cs+ and Ba2+, although the inhibitory manner of Cs+ and Ba2+ were voltage-dependent and voltage-independent, respectively. By the use of puff-application method, the local application of Ba2+ to basolateral surface of OHC shifted the holding current level in an inward direction, whereas the application to apex and hair showed little change. Indicating that the IK,n channels preferentially locate at the basolateral region of cell membrane.
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U2 - 10.1016/0006-8993(94)91207-6
DO - 10.1016/0006-8993(94)91207-6
M3 - Article
C2 - 7834381
AN - SCOPUS:0028171563
SN - 0006-8993
VL - 661
SP - 293
EP - 297
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1-2
ER -