Ionic properties of IK,n in outer hair cells of guinea pig cochlea

Takashi Nakagawa, Seiji Kakehata, Takehito Yamamoto, Norio Akaike, Shizuo Komune, Takuya Uemura

研究成果: ジャーナルへの寄稿学術誌査読

17 被引用数 (Scopus)


The ionic properties of voltage-dependent K+ current activated at the resting membrane potential (IK,n) of outer hair cells (OHC) isolated from the guinea pig cochlea were studied using a patch-clamp technique in a whole-cell recording mode. The reversal potential of IK,n indicated a high selectivity for K+, and the relative permeability ratios for various monovalent cations were K+ : Rb+ : NH+4 = 1 : 1.21 : 0.13. Decrease in extracellular Cl-1 inhibited the IK,n. IK,n was blocked by Cs+ and Ba2+, although the inhibitory manner of Cs+ and Ba2+ were voltage-dependent and voltage-independent, respectively. By the use of puff-application method, the local application of Ba2+ to basolateral surface of OHC shifted the holding current level in an inward direction, whereas the application to apex and hair showed little change. Indicating that the IK,n channels preferentially locate at the basolateral region of cell membrane.

ジャーナルBrain Research
出版ステータス出版済み - 10月 24 1994

!!!All Science Journal Classification (ASJC) codes

  • 神経科学(全般)
  • 分子生物学
  • 臨床神経学
  • 発生生物学


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