IRF8 Is a Critical Transcription Factor for Transforming Microglia into a Reactive Phenotype

Takahiro Masuda, Makoto Tsuda, Ryohei Yoshinaga, Hidetoshi Tozaki-Saitoh, Keiko Ozato, Tomohiko Tamura, Kazuhide Inoue

研究成果: ジャーナルへの寄稿学術誌査読

184 被引用数 (Scopus)

抄録

Microglia become activated by multiple types of damage in the nervous system and play essential roles in neuronal pathologies. However, how microglia transform into reactive phenotypes is poorly understood. Here, we identify the transcription factor interferon regulatory factor 8 (IRF8) as a critical regulator of reactive microglia. Within the spinal cord, IRF8 expression was normally low; however, the expression was markedly upregulated in microglia, but not in neurons or astrocytes, after peripheral nerve injury (PNI). IRF8 overexpression in cultured microglia promoted the transcription of genes associated with reactive states; conversely, IRF8 deficiency prevented these gene expressions in the spinal cord following PNI. Furthermore, IRF8-deficient mice were resistant to neuropathic pain, a common sequela of PNI, and transferring IRF8-overexpressing microglia spinally to normal mice produced pain. Therefore, IRF8 may activate a program of gene expression that transforms microglia into a reactive phenotype. Our findings provide a newly observed mechanism for microglial activation.

本文言語英語
ページ(範囲)334-340
ページ数7
ジャーナルCell Reports
1
4
DOI
出版ステータス出版済み - 3月 19 2012

!!!All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)

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