Ischemic preconditioning and remote ischemic preconditioning have protective effect against cold ischemia-reperfusion injury of rat small intestine

Isamu Saeki, Toshiharu Matsuura, Makoto Hayashida, Tomoaki Taguchi

研究成果: Contribution to journalArticle査読

26 被引用数 (Scopus)

抄録

Purpose: To investigate the protective effect of ischemic preconditioning (IPC) and remote ischemic preconditioning (RIPC) against cold ischemia-reperfusion injury (IRI) associated with small bowel transplantation (SBT). Methods: Male Lewis rats weighing 200-300 g were used for this study. The rats were assigned to three groups: control, ischemic preconditioning (IPC), or remote ischemic preconditioning (RIPC). Heterotopic SBT was thereafter performed. The recipient rats were killed 3, 6, 12 and 24 h after transplantation. Specimens from the intestine were histologically scored according to a grading system (Park et al.). Serum lactate dehydrogenase (LDH), aspirate aminotransferase (AST), alanine aminotransferase (ALT) were examined and heme oxygenase-1 (HO-1) were analyzed by ELISA where HO-1 served as an indicator of protection against IRI. Results: The values of tissue injury were significantly lower in the IPC and RIPC groups than in control group at 3 h after SBT. The serum LDH, AST and ALT levels also significantly decreased in the IPC and RIPC groups at 3 h after SBT, but these protective effects against cold IRI diminished by 12 and 24 h after SBT. The serum HO-1 level significantly increased in the IPC and RIPC groups 3 h after SBT. Conclusion: Both IPC and RIPC were found to ameliorate ischemia-reperfusion injury after rat SBT in the early phase. HO-1 may therefore play a protective role against cold IRI.

本文言語英語
ページ(範囲)857-862
ページ数6
ジャーナルPediatric surgery international
27
8
DOI
出版ステータス出版済み - 8 1 2011

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health
  • Surgery

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