抄録
Time-course analysis of single cells is important to characterize heterogeneous activities of individual cells such as the metabolic response to their environment. Single-cell isolation is an essential step prior to time-course analysis of individual cells by collecting, culturing, and identifying multiple single-cell targets. Although single-cell isolation has been performed by various methods previously, a glass microfluidic device with semiclosed microchannels dramatically improved this process with its simple operation and easy transfer for time-course analysis of identified single cells. This study demonstrates isolating single cells of the highly motile microalgae, Euglena gracilis, by semiclosed microchannels with liquid flow only. The isolated single cells were identified in isolating channels and continuously cultured to track, by Raman microscopy, for the formation of subcellular granules composed of polysaccharide paramylon, a unique metabolite of E. gracilis, generated through photosynthesis. Through low-temperature glass bonding, a thin glass interface was incorporated to the microfluidic device. Thus, the device could perform the direct measurements of cultured single cells at high magnification by Raman microscopy with low background noise. In this study, the first demonstration of sequential monitoring of paramylon biogenesis in a single identified E. gracilis cell is shown.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 9631-9639 |
| ページ数 | 9 |
| ジャーナル | Analytical chemistry |
| 巻 | 91 |
| 発行部数 | 15 |
| DOI | |
| 出版物ステータス | 出版済み - 8 6 2019 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
これを引用
Isolating Single Euglena gracilis Cells by Glass Microfluidics for Raman Analysis of Paramylon Biogenesis. / Ota, Nobutoshi; Yonamine, Yusuke; Asai, Takuya; Yalikun, Yaxiaer; Ito, Takuro; Ozeki, Yasuyuki; Hoshino, Yu; Tanaka, Yo.
：: Analytical chemistry, 巻 91, 番号 15, 06.08.2019, p. 9631-9639.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Isolating Single Euglena gracilis Cells by Glass Microfluidics for Raman Analysis of Paramylon Biogenesis
AU - Ota, Nobutoshi
AU - Yonamine, Yusuke
AU - Asai, Takuya
AU - Yalikun, Yaxiaer
AU - Ito, Takuro
AU - Ozeki, Yasuyuki
AU - Hoshino, Yu
AU - Tanaka, Yo
PY - 2019/8/6
Y1 - 2019/8/6
N2 - Time-course analysis of single cells is important to characterize heterogeneous activities of individual cells such as the metabolic response to their environment. Single-cell isolation is an essential step prior to time-course analysis of individual cells by collecting, culturing, and identifying multiple single-cell targets. Although single-cell isolation has been performed by various methods previously, a glass microfluidic device with semiclosed microchannels dramatically improved this process with its simple operation and easy transfer for time-course analysis of identified single cells. This study demonstrates isolating single cells of the highly motile microalgae, Euglena gracilis, by semiclosed microchannels with liquid flow only. The isolated single cells were identified in isolating channels and continuously cultured to track, by Raman microscopy, for the formation of subcellular granules composed of polysaccharide paramylon, a unique metabolite of E. gracilis, generated through photosynthesis. Through low-temperature glass bonding, a thin glass interface was incorporated to the microfluidic device. Thus, the device could perform the direct measurements of cultured single cells at high magnification by Raman microscopy with low background noise. In this study, the first demonstration of sequential monitoring of paramylon biogenesis in a single identified E. gracilis cell is shown.
AB - Time-course analysis of single cells is important to characterize heterogeneous activities of individual cells such as the metabolic response to their environment. Single-cell isolation is an essential step prior to time-course analysis of individual cells by collecting, culturing, and identifying multiple single-cell targets. Although single-cell isolation has been performed by various methods previously, a glass microfluidic device with semiclosed microchannels dramatically improved this process with its simple operation and easy transfer for time-course analysis of identified single cells. This study demonstrates isolating single cells of the highly motile microalgae, Euglena gracilis, by semiclosed microchannels with liquid flow only. The isolated single cells were identified in isolating channels and continuously cultured to track, by Raman microscopy, for the formation of subcellular granules composed of polysaccharide paramylon, a unique metabolite of E. gracilis, generated through photosynthesis. Through low-temperature glass bonding, a thin glass interface was incorporated to the microfluidic device. Thus, the device could perform the direct measurements of cultured single cells at high magnification by Raman microscopy with low background noise. In this study, the first demonstration of sequential monitoring of paramylon biogenesis in a single identified E. gracilis cell is shown.
UR - http://www.scopus.com/inward/record.url?scp=85071271946&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85071271946&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.9b01007
DO - 10.1021/acs.analchem.9b01007
M3 - Article
C2 - 31282650
AN - SCOPUS:85071271946
VL - 91
SP - 9631
EP - 9639
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 15
ER -