Isolation and characterization of a cDNA coding cowpea (Vigna unguiculata (L.) Walp.) calcineurin B-like protein-interacting protein kinase, VuCIPK1

Masakazu Imamura, Takashi Yuasa, Tomoko Takahashi, Nagisa Nakamura, Nang Myin Phyu Sin Htwe, Shao Hui Zheng, Ken Ichiro Shimazaki, Mari Iwaya-Inoue

研究成果: Contribution to journalArticle査読

12 被引用数 (Scopus)

抄録

We isolated a calcineurin B-like protein (CBL)-interacting protein kinase (EC 2.7.11.1) homolog (VuCIPK1) from cowpea (Vigna unguiculata (L.) Walp.) with significant similarity to AtCIPK3 (86% amino acid identity), which is involved in K+ transport. VuCIPK1 mRNA was detected in the whole plant by RT-PCR. Any significant change in VuCIPK1 mRNA levels was not detected when cowpea was subjected to various environmental stresses. Immunoblot assay with an anti-VuCIPK1 specific antibody and an anti-CBL antibody showed that immunologically CIPK- and CBL-related polypeptides were preferentially associated with membrane fractions of cowpea. The anti-VuCIPK1 antibody cross-reacted to two different CIPK-related polypeptides with relative molecular masses of 51 kDa in root and 55 kDa in mature leaf. Immunoprecipitation assay by anti-VuCIPK1 antibody using cowpea leaf extracts showed that endogenous VuCIPK1 was autophosphorylated on the threonine residues in response to salt stress. The recombinant VuCIPK1 phosphorylated casein in the presence of Mn 2+. These observations suggest that membrane-associated VuCIPK1 is involved osmotic stress-activated kinase regulated by its phosphorylation status.

本文言語英語
ページ(範囲)437-445
ページ数9
ジャーナルPlant Biotechnology
25
5
DOI
出版ステータス出版済み - 2008

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

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