Isolation and characterization of a new facultatively autotrophic hydrogen-oxidizing Betaproteobacterium, Hydrogenophaga sp. AH-24

Ki Seok Yoon, Natsuki Tsukada, Yukiko Sakai, Masaharu Ishii, Yasuo Igarashi, Hirofumi Nishihara

研究成果: Contribution to journalArticle査読

35 被引用数 (Scopus)

抄録

A hydrogen-oxidizing bacterium strain AH-24 was isolated, which was classified in the genus Hydrogenophaga, based on the 16S rRNA gene sequence. The isolate possessed a typical yellow pigment of Hydrogenophaga species. Its closest relative was Hydrogenophaga pseudoflava, but the assimilation profile of sugar compounds resembled that of no species of Hydrogenophaga. The optimum temperature and pH for autotrophic growth were, respectively, 33-35°C and 7.0. Most hydrogenase activity (benzyl viologen reducing activity) was localized in the membrane fraction (MF), but NAD(P)-reducing hydrogenase activity was detected in neither the membrane nor the soluble fractions. Cytochromes b 561 and c551 were present in MF; both were reduced when hydrogen was supplied to the oxidized MF, suggesting involvement in respiratory H2 oxidation as electron carriers. Cytochrome b561 was inferred to function as the redox partner of the membrane-bound hydrogenase.

本文言語英語
ページ(範囲)94-100
ページ数7
ジャーナルFEMS microbiology letters
278
1
DOI
出版ステータス出版済み - 1 1 2008
外部発表はい

All Science Journal Classification (ASJC) codes

  • 微生物学
  • 分子生物学
  • 遺伝学

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