Isolation and characterization of a novel F-box protein Pof10 in fission yeast

Chiho Ikebe, Kei Ichi Nakayama, Chiho Ikebe, Kin ichiro Kominami, Kei Ichi Nakayama, Kin ichiro Kominami, Kei Ichi Nakayama, Takashi Toda

研究成果: ジャーナルへの寄稿記事

9 引用 (Scopus)

抄録

The SCF complex is a type of ubiquitin-protein ligase (E3) that consists of invariable components, including Skp1, Cdc53/Cul1, and Rbx1, as well as variable components known as F-box proteins. Using a yeast two-hybrid system, we isolated six proteins that interact with Schizosaccharomyces pombe Skp1. Among them, Pof10 is a novel F-box protein consisting of 662 amino acids, harboring the F-box domain required for the binding to Skp1 and followed by four WD40 repeats. Overexpression of Pof10 in fission yeast resulted in loss of viability with marked morphological changes that are similar to those in pop1 mutant yeast. Coexpression of Skp1 with Pof10 prevented the lethality, suggesting that the lethality from Pof10 overexpression results from the sequestration of Skp1 from other F-box proteins including Pop1. Whereas most F-box proteins show rapid turnover, Pof10 has a remarkably long half-life in vivo and has been shown to be localized predominantly in cytoplasm. These results suggest that the stable F-box protein Pof10 might target abundant cytoplasmic proteins for degradation in fission yeast.

元の言語英語
ページ(範囲)1399-1407
ページ数9
ジャーナルBiochemical and Biophysical Research Communications
290
発行部数5
DOI
出版物ステータス出版済み - 1 1 2002

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F-Box Proteins
Schizosaccharomyces
Yeast
Two-Hybrid System Techniques
Ubiquitin-Protein Ligases
Hybrid systems
Proteolysis
Half-Life
Cytoplasm
Proteins
Yeasts
Amino Acids
Degradation

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Isolation and characterization of a novel F-box protein Pof10 in fission yeast. / Ikebe, Chiho; Nakayama, Kei Ichi; Ikebe, Chiho; Kominami, Kin ichiro; Nakayama, Kei Ichi; Kominami, Kin ichiro; Nakayama, Kei Ichi; Toda, Takashi.

:: Biochemical and Biophysical Research Communications, 巻 290, 番号 5, 01.01.2002, p. 1399-1407.

研究成果: ジャーナルへの寄稿記事

Ikebe, C, Nakayama, KI, Ikebe, C, Kominami, KI, Nakayama, KI, Kominami, KI, Nakayama, KI & Toda, T 2002, 'Isolation and characterization of a novel F-box protein Pof10 in fission yeast', Biochemical and Biophysical Research Communications, 巻. 290, 番号 5, pp. 1399-1407. https://doi.org/10.1006/bbrc.2002.6344
Ikebe, Chiho ; Nakayama, Kei Ichi ; Ikebe, Chiho ; Kominami, Kin ichiro ; Nakayama, Kei Ichi ; Kominami, Kin ichiro ; Nakayama, Kei Ichi ; Toda, Takashi. / Isolation and characterization of a novel F-box protein Pof10 in fission yeast. :: Biochemical and Biophysical Research Communications. 2002 ; 巻 290, 番号 5. pp. 1399-1407.
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