Isolation and characterization of cytochrome b-562 from cytochrome b-c1 comlex of the photosynthetic bacterium Rhodopseudomonas sphaeroides R-26

Koh Iba, Ken ichiro Takamiya, Hiroyuki Arata

研究成果: Contribution to journalArticle査読

12 被引用数 (Scopus)

抄録

A b-type cytochrome (cytochrome b-562) was highly purified from the isolated cytochrome b-c1 complex of Rhodopseudomonas sphaeroides R-26 by treatment with sodium N-lauroyl sarcosinate (sarkosyi) and sodium thiocyanate, followed by gel filtration. The purified cytochrome b-562 was electrophoretically homogeneous, and its minimal Mr was estimated to be 16000 by SDS-polyacrylamide gel electrophoresis. It showed absorption maxima at 278, 415 and 534 nm in the oxidized form, and 428, 530 and 562 nm in the reduced form. Redox titration of this cytochrome was biphasic, giving two midpoint potentials, 55 and -100 mV, at pH 7.0. This indicates that the cytochrome is intact. Cytochrome b-562 Cytochrome b-C1 complex Rhodopseudomonas sphaeroides.

本文言語英語
ページ(範囲)151-154
ページ数4
ジャーナルFEBS Letters
183
1
DOI
出版ステータス出版済み - 4 8 1985

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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