Isolation and characterization of microsatellite loci from Larix kaempferi

Keiya Isoda, Atsushi Watanabe

研究成果: ジャーナルへの寄稿記事

30 引用 (Scopus)

抄録

Microsatellites were isolated and characterized for Japanese larch, Larix kaempferi, a conifer species distributed in Japan. A larch genomic DNA library enriched for (AG)n repeats was screened using the colony polymerase chain reaction method and 145 unique microsatellite containing sequences were obtained. Seventy-two primer pairs were designed and 30 produced single-locus products, and 19 of them were polymorphic. The expected heterozygosity ranged from 0.566 to 0.951. These 19 polymorphic microsatellite loci should be valuable markers for genetic studies on Japanese larch.

元の言語英語
ページ(範囲)664-666
ページ数3
ジャーナルMolecular Ecology Notes
6
発行部数3
DOI
出版物ステータス出版済み - 9 2006
外部発表Yes

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Larix
Larix kaempferi
heterozygosity
Microsatellite Repeats
polymerase chain reaction
coniferous tree
genomics
microsatellite repeats
DNA
loci
DNA libraries
Polymerase chain reaction
Coniferophyta
Gene Library
conifers
Genomic Library
Genetic Markers
Japan
genetic markers
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

これを引用

Isolation and characterization of microsatellite loci from Larix kaempferi. / Isoda, Keiya; Watanabe, Atsushi.

:: Molecular Ecology Notes, 巻 6, 番号 3, 09.2006, p. 664-666.

研究成果: ジャーナルへの寄稿記事

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