Isolation and characterization of microsatellite loci from Larix kaempferi

Keiya Isoda, Atsushi Watanabe

研究成果: Contribution to journalArticle査読

32 被引用数 (Scopus)

抄録

Microsatellites were isolated and characterized for Japanese larch, Larix kaempferi, a conifer species distributed in Japan. A larch genomic DNA library enriched for (AG)n repeats was screened using the colony polymerase chain reaction method and 145 unique microsatellite containing sequences were obtained. Seventy-two primer pairs were designed and 30 produced single-locus products, and 19 of them were polymorphic. The expected heterozygosity ranged from 0.566 to 0.951. These 19 polymorphic microsatellite loci should be valuable markers for genetic studies on Japanese larch.

本文言語英語
ページ(範囲)664-666
ページ数3
ジャーナルMolecular Ecology Notes
6
3
DOI
出版ステータス出版済み - 9 2006
外部発表はい

All Science Journal Classification (ASJC) codes

  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

フィンガープリント 「Isolation and characterization of microsatellite loci from Larix kaempferi」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル