Isolation and characterization of novobiocin-resistant BHK cells

Ryoji Ishida, Miwako Nishizawa, Katsuya Fukami, Ko Maekawa, Taijo Takahashi, Takeharu Nishimoto

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

We isolated two novobiocin-resistant mutants which were stable and approximately three and four times more resistant than the parent cells to novobiocin. Both mutants (Novr A2, Novr) A41 were more sensitive than the wild-type cells to nalidixic acid, and cold sensitive for cell growth. When we isolated derivatives of Novr A2 and Novr A41 cells which are resistant to nalidixic acid, those are found to be phenotypically reverted to novobiocin sensitivity like wild-type cells, thereby suggesting the relationship between the targets for novobiocin and for nalidixic acid. But the cold sensitivity did not always revert to wild type, with accompanying resistance to nalidixic acid. The DNA and RNA syntheses of Novr mutants were more resistant to novobiocin but more sensitive to nalidixic acid, than those of wild-type cells. However, in vitro assays of wild-type and Novr cell extracts were unable to demonstrate any differences in the sensitivity of topoisomerase II activity to inhibition by novobiocin. While the targets of novobiocin and nalidixic acid show a mutual interaction in vivo and play a role in DNA replication and transcription, our results suggest that these targets are probably not topoisomerase II.

元の言語英語
ページ(範囲)11-20
ページ数10
ジャーナルSomatic Cell and Molecular Genetics
13
発行部数1
DOI
出版物ステータス出版済み - 1 1 1987

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Novobiocin
Nalidixic Acid
Type II DNA Topoisomerase
varespladib methyl
Cell Extracts
DNA Replication
RNA
DNA
Growth

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

これを引用

Ishida, R., Nishizawa, M., Fukami, K., Maekawa, K., Takahashi, T., & Nishimoto, T. (1987). Isolation and characterization of novobiocin-resistant BHK cells. Somatic Cell and Molecular Genetics, 13(1), 11-20. https://doi.org/10.1007/BF02422295

Isolation and characterization of novobiocin-resistant BHK cells. / Ishida, Ryoji; Nishizawa, Miwako; Fukami, Katsuya; Maekawa, Ko; Takahashi, Taijo; Nishimoto, Takeharu.

:: Somatic Cell and Molecular Genetics, 巻 13, 番号 1, 01.01.1987, p. 11-20.

研究成果: ジャーナルへの寄稿記事

Ishida, R, Nishizawa, M, Fukami, K, Maekawa, K, Takahashi, T & Nishimoto, T 1987, 'Isolation and characterization of novobiocin-resistant BHK cells', Somatic Cell and Molecular Genetics, 巻. 13, 番号 1, pp. 11-20. https://doi.org/10.1007/BF02422295
Ishida, Ryoji ; Nishizawa, Miwako ; Fukami, Katsuya ; Maekawa, Ko ; Takahashi, Taijo ; Nishimoto, Takeharu. / Isolation and characterization of novobiocin-resistant BHK cells. :: Somatic Cell and Molecular Genetics. 1987 ; 巻 13, 番号 1. pp. 11-20.
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