Isolation and characterization of porcine endothelin-like peptide

Osamu Nakagawa, Kazuwa Nakao, Yoshihiko Saito, Gotaro Shirakami, Michihisa Jougasaki, Masashi Mukoyama, Kiminori Hosoda, Shin ichi Suga, Yoshihiro Ogawa, Ichiro Kishimoto, Yasato Komatsu, Norio Hama, Hiroo Imura

研究成果: Contribution to journalArticle査読


Two endogenous molecules possessing an endothelin-like sequence with an apparent molecular weight of 7 kDa were isolated from serum-free culture medium of porcine aortic endothelial cells, using a specific radioimmunoassay. N-terminal sequences of two molecules were identical to each other and were Ser-Leu-Lys-Asp-Leu-Phe-Pro-Ala-Lys-Ala-Ala-Asp-Arg-Arg-Asp-Arg-X-Gln-X-Ala-X-Gln-Lys-Asp, which corresponds to the sequence [94-117] of preproendothelin-1. This finding indicates that these two molecules may be closely related peptides such as the oxidized form or disulfide analogues and also suggests that the endogenous peptide possessing an endothelin-like sequence is generated by proteolytic cleavage at paired basic amino acids Arg92-Arg93Further studies on the structure and function of new endogenous peptides possessing an endothelin-like sequence are ongoing in our laboratory.

ジャーナルJournal of Cardiovascular Pharmacology
出版ステータス出版済み - 1991

All Science Journal Classification (ASJC) codes

  • 薬理学
  • 循環器および心血管医学


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