We isolated over 20 phage clones carrying the ornithine transcarbamylase (OTC) [EC 188.8.131.52] gene, from two independently constructed human genomic DNA libraries, using as probes either a rat OTC eDNA or several nuclear DNA fragments derived from some of these isolated clones. These clones, classified into 10 different groups, overlapped and spanned a region of more than 85 kilobase pairs of the human genomic DNA. Restriction mapping and Southern blot analyses demonstrated that one of the clones covers the 5′-end region of the OTC gene. We sequenced the 5′-end region of the OTC gene and found that it covered 665 base pairs of the 5′-flanking region, the complete first exon and a part of the first intron (150 base pairs). In the 5′-flanking region, there were two pairs of putative CAAT and TATA boxes and one enhancer core-like sequence, GTGGAAAG. The first exon contained a coding region for most of the OTC presequence, i.e. 26 out of 32 amino acid residues of the presequence, including the initiation methionine.
|ジャーナル||Journal of Biochemistry|
|出版ステータス||出版済み - 1月 1 1986|
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