Isolation and characterization of the mouse CD8 β-chain (Ly-3) genes. Absenced of an intervening sequence between V- and J-like gene segments

K. I. Nakayama, Y. I. Shinkai, K. Okumura, H. Nakauchi

研究成果: ジャーナルへの寄稿学術誌査読

18 被引用数 (Scopus)

抄録

We have isolated and determined the nucleotide sequence and genomic organization of the genes encoding Ly-3.1 and Ly-3.2. These genes span approximately 14 kb on chromosome 6 and consist of six exons and five introns. The exons correlate roughly with the putative functional domains, namely, a leader exon, a variable and joining region-like exon, a hinge region-like exon, a transmembrane exon, and two intracytoplasmic exons. There is no intervening sequence between V- and J-like gene segments, indicating that rearrangement is not necessary for the expression of the Ly-3 gene. In the 5'-flanking region there is no'TATA' box nor 'CAAT' box; however, three 'GC' boxes are located upstream of the ATG initiator codon. There are short stretches of sequence homologous to 5'-flanking sequences of the Ly-2 gene. In addition, the sequences CTCTGTGGCA at -748 exhibits homology to the enhancer core sequence of the human Ig H chain and TCR genes. Comparison of the nucleotide sequence corresponding to the extracellular portion between Ly-3.1 and Ly-3.2 revealed a single base difference which results in an amino acid substitution. Therefore it is likely that this amino acid difference is responsible for the previously defined Ly-3 allotypes.

本文言語英語
ページ(範囲)2540-2546
ページ数7
ジャーナルJournal of Immunology
142
7
出版ステータス出版済み - 1月 1 1989
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 免疫アレルギー学
  • 免疫学

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