Isolation and Characterization of Two β-D-Glucosidases from Bifidobacterium breve 203

Kenji Sakai, Takashi Tachiki, Hidehiko Kumagai, Tatsurokuro Tochikura

研究成果: Contribution to journalArticle査読

8 被引用数 (Scopus)

抄録

Two β-D-glucosidases were purified to homogeneity from Bifidobacterium breve 203: one β-D-glucosidase I; molecular weight, 96,000) showed reactivity toward p-nitrophenyl O-NP) P-D-fucoside, 74% of that to p-NP β-D-glucoside, and the other (β-D-glucosidase II; molecular weight, 450,000) did not. They also differed in their thermal and pH stabilities. Laminaribiose, cellobiose and gentiobiose were hydrolyzed by β-D-glucosidase I, with 53%, 34% and 3% of the reactivity in the case of p-NP β-D-glucoside, and by β-D-glucosidase II, with 53%, 6% and 107% of the reactivity. The reaction of β-D-glucosidase I with p-NP β-D-fucoside was enhanced by the addition of glucose and other monosaccharides to the reaction mixture, whereas that with p-NP β-D-glucoside was not affected. The activity of β-D-glucosidase II with p-NP β-D-glucoside was inhibited by glucose.

本文言語英語
ページ(範囲)2287-2293
ページ数7
ジャーナルAgricultural and Biological Chemistry
50
9
DOI
出版ステータス出版済み - 1986
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)

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