Isolation and partial characterization of a 39 kDa major outer membrane protein of Actinobacillus actinomycetemcomitans Y4

Susumu Kokeguchi, Keijiro Kato, Fusanori Nishimura, Hidemi Kurihara, Yoji Murayama

研究成果: Contribution to journalArticle査読

13 被引用数 (Scopus)

抄録

The outer membrane fractions of Actinobacillus actinomycetemcomitans, which were extracted from whole cells with cetyl trimethyl ammonium bromide and CaCl2, contained four major outer membrane proteins (MOMP) of 39, 37, 36 and 30 kDa. The 39 kDa MOMP of A. actinomycetemcomitans was sequentially purified by extraction with Zwittergent 3-14 detergent, anion-exchange chromatography and gel filtration chromatography. Analysis of amino acid composition and N-terminal amino acid sequence of 20 residues of purified 39 kDa MOMP was performed. Although some of the periodontitis patient sera reacted strongly with 39 kDa and 30 kDa MOMP in crude outer membrane fractions, purified 39 kDa MOMP showed decreased immunoreactivity with the human sera.

本文言語英語
ページ(範囲)85-90
ページ数6
ジャーナルFEMS microbiology letters
77
1
DOI
出版ステータス出版済み - 1 1 1991
外部発表はい

All Science Journal Classification (ASJC) codes

  • 微生物学
  • 分子生物学
  • 遺伝学

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