@inbook{e80507fa1c384e71a22146db87c90063,
title = "Isolation and purification of satellite cells from young rats by percoll density gradient centrifugation",
abstract = "Satellite cells (SCs) are myogenic stem cells that play an important role in skeletal muscle regeneration and hypertrophy. Primary cultures of SCs are useful to analyze cell functions; however, it is difficult to obtain highly pure SCs from young rats with the conventional procedures. The purpose of this study is to establish a purification method for SC isolation from young rats and quantitatively evaluate the purification procedure employing Percoll, a common research tool to purify cells. We elucidated the purity of SCs collected by Percoll density gradient centrifugation using real-time RT-qPCR and immunocytochemistry for desmin. Percoll treatment increased the purity of SCs isolated from young rats to nearly 90%, which was comparable to that achieved with the conventional method using middle-aged rats.",
author = "Yuji Matsuyoshi and Mariko Akahoshi and Mako Nakamura and Ryuichi Tatsumi and Wataru Mizunoya",
year = "2019",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-8897-6_6",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "81--93",
booktitle = "Methods in Molecular Biology",
}