Isolation and purification of satellite cells from young rats by percoll density gradient centrifugation

Yuji Matsuyoshi, Mariko Akahoshi, Mako Nakamura, Ryuichi Tatsumi, Wataru Mizunoya

研究成果: 著書/レポートタイプへの貢献

2 引用 (Scopus)

抄録

Satellite cells (SCs) are myogenic stem cells that play an important role in skeletal muscle regeneration and hypertrophy. Primary cultures of SCs are useful to analyze cell functions; however, it is difficult to obtain highly pure SCs from young rats with the conventional procedures. The purpose of this study is to establish a purification method for SC isolation from young rats and quantitatively evaluate the purification procedure employing Percoll, a common research tool to purify cells. We elucidated the purity of SCs collected by Percoll density gradient centrifugation using real-time RT-qPCR and immunocytochemistry for desmin. Percoll treatment increased the purity of SCs isolated from young rats to nearly 90%, which was comparable to that achieved with the conventional method using middle-aged rats.

元の言語英語
ホスト出版物のタイトルMethods in Molecular Biology
出版者Humana Press Inc.
ページ81-93
ページ数13
DOI
出版物ステータス出版済み - 1 1 2019

出版物シリーズ

名前Methods in Molecular Biology
1889
ISSN(印刷物)1064-3745

Fingerprint

Density Gradient Centrifugation
Skeletal Muscle Satellite Cells
Primary Cell Culture
Desmin
Cell Separation
Hypertrophy
Regeneration
Skeletal Muscle
Stem Cells
Immunohistochemistry
Percoll
Research

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

これを引用

Matsuyoshi, Y., Akahoshi, M., Nakamura, M., Tatsumi, R., & Mizunoya, W. (2019). Isolation and purification of satellite cells from young rats by percoll density gradient centrifugation. : Methods in Molecular Biology (pp. 81-93). (Methods in Molecular Biology; 巻数 1889). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8897-6_6

Isolation and purification of satellite cells from young rats by percoll density gradient centrifugation. / Matsuyoshi, Yuji; Akahoshi, Mariko; Nakamura, Mako; Tatsumi, Ryuichi; Mizunoya, Wataru.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 81-93 (Methods in Molecular Biology; 巻 1889).

研究成果: 著書/レポートタイプへの貢献

Matsuyoshi, Y, Akahoshi, M, Nakamura, M, Tatsumi, R & Mizunoya, W 2019, Isolation and purification of satellite cells from young rats by percoll density gradient centrifugation. : Methods in Molecular Biology. Methods in Molecular Biology, 巻. 1889, Humana Press Inc., pp. 81-93. https://doi.org/10.1007/978-1-4939-8897-6_6
Matsuyoshi Y, Akahoshi M, Nakamura M, Tatsumi R, Mizunoya W. Isolation and purification of satellite cells from young rats by percoll density gradient centrifugation. : Methods in Molecular Biology. Humana Press Inc. 2019. p. 81-93. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8897-6_6
Matsuyoshi, Yuji ; Akahoshi, Mariko ; Nakamura, Mako ; Tatsumi, Ryuichi ; Mizunoya, Wataru. / Isolation and purification of satellite cells from young rats by percoll density gradient centrifugation. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 81-93 (Methods in Molecular Biology).
@inbook{e80507fa1c384e71a22146db87c90063,
title = "Isolation and purification of satellite cells from young rats by percoll density gradient centrifugation",
abstract = "Satellite cells (SCs) are myogenic stem cells that play an important role in skeletal muscle regeneration and hypertrophy. Primary cultures of SCs are useful to analyze cell functions; however, it is difficult to obtain highly pure SCs from young rats with the conventional procedures. The purpose of this study is to establish a purification method for SC isolation from young rats and quantitatively evaluate the purification procedure employing Percoll, a common research tool to purify cells. We elucidated the purity of SCs collected by Percoll density gradient centrifugation using real-time RT-qPCR and immunocytochemistry for desmin. Percoll treatment increased the purity of SCs isolated from young rats to nearly 90{\%}, which was comparable to that achieved with the conventional method using middle-aged rats.",
author = "Yuji Matsuyoshi and Mariko Akahoshi and Mako Nakamura and Ryuichi Tatsumi and Wataru Mizunoya",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-8897-6_6",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "81--93",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Isolation and purification of satellite cells from young rats by percoll density gradient centrifugation

AU - Matsuyoshi, Yuji

AU - Akahoshi, Mariko

AU - Nakamura, Mako

AU - Tatsumi, Ryuichi

AU - Mizunoya, Wataru

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Satellite cells (SCs) are myogenic stem cells that play an important role in skeletal muscle regeneration and hypertrophy. Primary cultures of SCs are useful to analyze cell functions; however, it is difficult to obtain highly pure SCs from young rats with the conventional procedures. The purpose of this study is to establish a purification method for SC isolation from young rats and quantitatively evaluate the purification procedure employing Percoll, a common research tool to purify cells. We elucidated the purity of SCs collected by Percoll density gradient centrifugation using real-time RT-qPCR and immunocytochemistry for desmin. Percoll treatment increased the purity of SCs isolated from young rats to nearly 90%, which was comparable to that achieved with the conventional method using middle-aged rats.

AB - Satellite cells (SCs) are myogenic stem cells that play an important role in skeletal muscle regeneration and hypertrophy. Primary cultures of SCs are useful to analyze cell functions; however, it is difficult to obtain highly pure SCs from young rats with the conventional procedures. The purpose of this study is to establish a purification method for SC isolation from young rats and quantitatively evaluate the purification procedure employing Percoll, a common research tool to purify cells. We elucidated the purity of SCs collected by Percoll density gradient centrifugation using real-time RT-qPCR and immunocytochemistry for desmin. Percoll treatment increased the purity of SCs isolated from young rats to nearly 90%, which was comparable to that achieved with the conventional method using middle-aged rats.

UR - http://www.scopus.com/inward/record.url?scp=85055612608&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055612608&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-8897-6_6

DO - 10.1007/978-1-4939-8897-6_6

M3 - Chapter

T3 - Methods in Molecular Biology

SP - 81

EP - 93

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -