抄録
Satellite cells (SCs) are myogenic stem cells that play an important role in skeletal muscle regeneration and hypertrophy. Primary cultures of SCs are useful to analyze cell functions; however, it is difficult to obtain highly pure SCs from young rats with the conventional procedures. The purpose of this study is to establish a purification method for SC isolation from young rats and quantitatively evaluate the purification procedure employing Percoll, a common research tool to purify cells. We elucidated the purity of SCs collected by Percoll density gradient centrifugation using real-time RT-qPCR and immunocytochemistry for desmin. Percoll treatment increased the purity of SCs isolated from young rats to nearly 90%, which was comparable to that achieved with the conventional method using middle-aged rats.
| 元の言語 | 英語 |
|---|---|
| ホスト出版物のタイトル | Methods in Molecular Biology |
| 出版者 | Humana Press Inc. |
| ページ | 81-93 |
| ページ数 | 13 |
| DOI | |
| 出版物ステータス | 出版済み - 1 1 2019 |
出版物シリーズ
| 名前 | Methods in Molecular Biology |
|---|---|
| 巻 | 1889 |
| ISSN（印刷物） | 1064-3745 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Genetics
これを引用
Isolation and purification of satellite cells from young rats by percoll density gradient centrifugation. / Matsuyoshi, Yuji; Akahoshi, Mariko; Nakamura, Mako; Tatsumi, Ryuichi; Mizunoya, Wataru.
Methods in Molecular Biology. Humana Press Inc., 2019. p. 81-93 (Methods in Molecular Biology; 巻 1889).研究成果: 著書/レポートタイプへの貢献 › 章
}
TY - CHAP
T1 - Isolation and purification of satellite cells from young rats by percoll density gradient centrifugation
AU - Matsuyoshi, Yuji
AU - Akahoshi, Mariko
AU - Nakamura, Mako
AU - Tatsumi, Ryuichi
AU - Mizunoya, Wataru
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Satellite cells (SCs) are myogenic stem cells that play an important role in skeletal muscle regeneration and hypertrophy. Primary cultures of SCs are useful to analyze cell functions; however, it is difficult to obtain highly pure SCs from young rats with the conventional procedures. The purpose of this study is to establish a purification method for SC isolation from young rats and quantitatively evaluate the purification procedure employing Percoll, a common research tool to purify cells. We elucidated the purity of SCs collected by Percoll density gradient centrifugation using real-time RT-qPCR and immunocytochemistry for desmin. Percoll treatment increased the purity of SCs isolated from young rats to nearly 90%, which was comparable to that achieved with the conventional method using middle-aged rats.
AB - Satellite cells (SCs) are myogenic stem cells that play an important role in skeletal muscle regeneration and hypertrophy. Primary cultures of SCs are useful to analyze cell functions; however, it is difficult to obtain highly pure SCs from young rats with the conventional procedures. The purpose of this study is to establish a purification method for SC isolation from young rats and quantitatively evaluate the purification procedure employing Percoll, a common research tool to purify cells. We elucidated the purity of SCs collected by Percoll density gradient centrifugation using real-time RT-qPCR and immunocytochemistry for desmin. Percoll treatment increased the purity of SCs isolated from young rats to nearly 90%, which was comparable to that achieved with the conventional method using middle-aged rats.
UR - http://www.scopus.com/inward/record.url?scp=85055612608&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85055612608&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-8897-6_6
DO - 10.1007/978-1-4939-8897-6_6
M3 - Chapter
C2 - 30367410
AN - SCOPUS:85055612608
T3 - Methods in Molecular Biology
SP - 81
EP - 93
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -