Isolation and purification of satellite cells from young rats by percoll density gradient centrifugation

Yuji Matsuyoshi, Mariko Akahoshi, Mako Nakamura, Ryuichi Tatsumi, Wataru Mizunoya

研究成果: Chapter in Book/Report/Conference proceedingChapter

5 引用 (Scopus)

抜粋

Satellite cells (SCs) are myogenic stem cells that play an important role in skeletal muscle regeneration and hypertrophy. Primary cultures of SCs are useful to analyze cell functions; however, it is difficult to obtain highly pure SCs from young rats with the conventional procedures. The purpose of this study is to establish a purification method for SC isolation from young rats and quantitatively evaluate the purification procedure employing Percoll, a common research tool to purify cells. We elucidated the purity of SCs collected by Percoll density gradient centrifugation using real-time RT-qPCR and immunocytochemistry for desmin. Percoll treatment increased the purity of SCs isolated from young rats to nearly 90%, which was comparable to that achieved with the conventional method using middle-aged rats.

元の言語英語
ホスト出版物のタイトルMethods in Molecular Biology
出版者Humana Press Inc.
ページ81-93
ページ数13
DOI
出版物ステータス出版済み - 1 1 2019

出版物シリーズ

名前Methods in Molecular Biology
1889
ISSN(印刷物)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

フィンガープリント Isolation and purification of satellite cells from young rats by percoll density gradient centrifugation' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Matsuyoshi, Y., Akahoshi, M., Nakamura, M., Tatsumi, R., & Mizunoya, W. (2019). Isolation and purification of satellite cells from young rats by percoll density gradient centrifugation. : Methods in Molecular Biology (pp. 81-93). (Methods in Molecular Biology; 巻数 1889). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8897-6_6