Isolation of a new peroxisome-deficient CHO cell mutant defective in peroxisome targeting signal-1 receptor

Toshiro Tsukamoto, Akemi Bogaki, Kanji Okumoto, Keita Tateishi, Yukio Fujiki, Nobuyuki Shimozawa, Yasuyuki Suzuki, Naomi Kondo, Takashi Osumi

研究成果: ジャーナルへの寄稿学術誌査読

36 被引用数 (Scopus)

抄録

For the study of mechanism of peroxisome biogenesis, we attempted to isolate CHO cell mutants deficient in peroxisome biogenesis. We used as the parent strain a stable CHO transformant of rat PEX2 (formerly named peroxisome assembly factor-1) cDNA, to avoid unusually frequent isolation of Pex2 mutants. Among the three peroxisome-deficient mutants obtained, ZP102 was a new CHO mutant of complementation group 2, and was restored for peroxisome assembly by the transfection of human PEX5 (formerly called PXR1 or PTS1R) cDNA. This approach would facilitate the isolation of new complementation gorups of peroxisome-deficient CHO mutants and the identification of essential genes for peroxisome biogenesis.

本文言語英語
ページ(範囲)402-406
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
230
2
DOI
出版ステータス出版済み - 1月 13 1997

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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