Isolation of a new peroxisome-deficient CHO cell mutant defective in peroxisome targeting signal-1 receptor

Toshiro Tsukamoto, Akemi Bogaki, Kanji Okumoto, Keita Tateishi, Yukio Fujiki, Nobuyuki Shimozawa, Yasuyuki Suzuki, Naomi Kondo, Takashi Osumi

研究成果: ジャーナルへの寄稿記事

34 引用 (Scopus)

抄録

For the study of mechanism of peroxisome biogenesis, we attempted to isolate CHO cell mutants deficient in peroxisome biogenesis. We used as the parent strain a stable CHO transformant of rat PEX2 (formerly named peroxisome assembly factor-1) cDNA, to avoid unusually frequent isolation of Pex2 mutants. Among the three peroxisome-deficient mutants obtained, ZP102 was a new CHO mutant of complementation group 2, and was restored for peroxisome assembly by the transfection of human PEX5 (formerly called PXR1 or PTS1R) cDNA. This approach would facilitate the isolation of new complementation gorups of peroxisome-deficient CHO mutants and the identification of essential genes for peroxisome biogenesis.

元の言語英語
ページ(範囲)402-406
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
230
発行部数2
DOI
出版物ステータス出版済み - 1 13 1997

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Peroxisomes
CHO Cells
Complementary DNA
Rats
Genes
Essential Genes
Transfection
peroxisome-targeting signal 1 receptor
peroxisome assembly factor-1

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Isolation of a new peroxisome-deficient CHO cell mutant defective in peroxisome targeting signal-1 receptor. / Tsukamoto, Toshiro; Bogaki, Akemi; Okumoto, Kanji; Tateishi, Keita; Fujiki, Yukio; Shimozawa, Nobuyuki; Suzuki, Yasuyuki; Kondo, Naomi; Osumi, Takashi.

:: Biochemical and Biophysical Research Communications, 巻 230, 番号 2, 13.01.1997, p. 402-406.

研究成果: ジャーナルへの寄稿記事

Tsukamoto, T, Bogaki, A, Okumoto, K, Tateishi, K, Fujiki, Y, Shimozawa, N, Suzuki, Y, Kondo, N & Osumi, T 1997, 'Isolation of a new peroxisome-deficient CHO cell mutant defective in peroxisome targeting signal-1 receptor', Biochemical and Biophysical Research Communications, 巻. 230, 番号 2, pp. 402-406. https://doi.org/10.1006/bbrc.1996.5971
Tsukamoto, Toshiro ; Bogaki, Akemi ; Okumoto, Kanji ; Tateishi, Keita ; Fujiki, Yukio ; Shimozawa, Nobuyuki ; Suzuki, Yasuyuki ; Kondo, Naomi ; Osumi, Takashi. / Isolation of a new peroxisome-deficient CHO cell mutant defective in peroxisome targeting signal-1 receptor. :: Biochemical and Biophysical Research Communications. 1997 ; 巻 230, 番号 2. pp. 402-406.
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AU - Fujiki, Yukio

AU - Shimozawa, Nobuyuki

AU - Suzuki, Yasuyuki

AU - Kondo, Naomi

AU - Osumi, Takashi

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