Isolation of carp complement factor B and formation of the C3-convertase

Miki Nakao, Makiko Nakahara, Tomoki Yano

研究成果: ジャーナルへの寄稿記事

抄録

Complement factor B (Bf) is a key component that forms a catalytic site of C3-convertase of the alternative activation pathway. However, the functional assay and isolation method has been developed poorly for Bf in fish. We describe here a hemolytic assay method for Bf of carp (Cyprinus carpio). The assay devised from purified carp C3 and factor D (Df) is based on the action of Bf which cleaves C3 in the presence of Df. Using this assay method as a probe, carp Bf was isolated from serum, and proved to be a protein composed of a single polypeptide with a molecular mass of 93 kDa. This Bf protein was identified as a mature product of the B/C2-A1 isotype gene of carp. The C3-convertase of the alternative pathway of carp complement was assembled from purified C3, Bf and Df, and generation of C3b, the initial biologically active fragment derived from C3, was confirmed, suggesting a well conserved mechanism of the formation of the alternative pathway C3-convertase.

元の言語英語
ページ(範囲)101-110
ページ数10
ジャーナルJournal of the Faculty of Agriculture, Kyushu University
49
発行部数1
出版物ステータス出版済み - 2 1 2004

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Complement C3-C5 Convertases
Complement Factor B
Carps
carp
Alternative Pathway Complement C3 Convertase
complement
assays
C3 photosynthesis
Complement Factor D
isolation techniques
Cyprinus carpio
active sites
probes (equipment)
polypeptides
proteins
Catalytic Domain
molecular weight
Fishes
Proteins
Peptides

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

これを引用

Isolation of carp complement factor B and formation of the C3-convertase. / Nakao, Miki; Nakahara, Makiko; Yano, Tomoki.

:: Journal of the Faculty of Agriculture, Kyushu University, 巻 49, 番号 1, 01.02.2004, p. 101-110.

研究成果: ジャーナルへの寄稿記事

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