Isolation of microsatellite markers from Pinus densiflora Sieb. et Zucc. using a dual PCR technique

A. Watanabe, M. G. Iwaizumi, M. Ubukata, T. Kondo, C. Lian, T. Hogetsu

研究成果: ジャーナルへの寄稿学術誌査読

16 被引用数 (Scopus)

抄録

We developed seven microsatellite loci from Pinus densiflora using a dual polymerase chain reaction (PCR) technique. Of 186 clones from a library based on suppression PCR, 127 contained microsatellite sequences. Of these, 43 candidates were determined sequences of both flanking regions, and 16 regions from this group were chosen as development markers. Seven of these primer pairs successfully amplified polymorphic single loci among 83 resistant trees against pine wood nematode. The observed heterozygosity of the seven microsatellite markers ranged from 0.247 to 0.843. Mendelian inheritance was confirmed using megagametophytes.

本文言語英語
ページ(範囲)80-82
ページ数3
ジャーナルMolecular Ecology Notes
6
1
DOI
出版ステータス出版済み - 3月 1 2006
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 生態学
  • 生化学、遺伝学、分子生物学(全般)

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