抄録
Two fragments that could function as replicational cue elements were isolated from a genomic DNA digest of Aspergillus oryzae on the basis of abnormal behavior in polyacrylamide gel electrophoresis. The vector used in this study contained a scaffold-associated region of the Drosophila melanogaster ftz gene to provide nuclear retention. Neither fragment contained a yeast ARS consensus sequence or an eukaryotic topoisomerase II binding sequence. One of the fragments showed sequence homology with the mitochondrial replication origin of Candida utilis and a portion of mitochondrial DNA of Aspergillus nidulans. This plasmid carrying the cue fragment could also replicate in HeLa and NIH3T3 cells.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 13-19 |
| ページ数 | 7 |
| ジャーナル | Molecular Biology Reports |
| 巻 | 27 |
| 発行部数 | 1 |
| DOI | |
| 出版物ステータス | 出版済み - 1 1 2000 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Genetics
これを引用
Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae. / Kusakabe, Takahiro; Sugimoto, Yasushi; Hirota, Yoshiko; Toné, Shigenobu; Kawaguchi, Yutaka; Koga, Katsumi; Ohyama, Takashi.
：: Molecular Biology Reports, 巻 27, 番号 1, 01.01.2000, p. 13-19.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae
AU - Kusakabe, Takahiro
AU - Sugimoto, Yasushi
AU - Hirota, Yoshiko
AU - Toné, Shigenobu
AU - Kawaguchi, Yutaka
AU - Koga, Katsumi
AU - Ohyama, Takashi
PY - 2000/1/1
Y1 - 2000/1/1
N2 - Two fragments that could function as replicational cue elements were isolated from a genomic DNA digest of Aspergillus oryzae on the basis of abnormal behavior in polyacrylamide gel electrophoresis. The vector used in this study contained a scaffold-associated region of the Drosophila melanogaster ftz gene to provide nuclear retention. Neither fragment contained a yeast ARS consensus sequence or an eukaryotic topoisomerase II binding sequence. One of the fragments showed sequence homology with the mitochondrial replication origin of Candida utilis and a portion of mitochondrial DNA of Aspergillus nidulans. This plasmid carrying the cue fragment could also replicate in HeLa and NIH3T3 cells.
AB - Two fragments that could function as replicational cue elements were isolated from a genomic DNA digest of Aspergillus oryzae on the basis of abnormal behavior in polyacrylamide gel electrophoresis. The vector used in this study contained a scaffold-associated region of the Drosophila melanogaster ftz gene to provide nuclear retention. Neither fragment contained a yeast ARS consensus sequence or an eukaryotic topoisomerase II binding sequence. One of the fragments showed sequence homology with the mitochondrial replication origin of Candida utilis and a portion of mitochondrial DNA of Aspergillus nidulans. This plasmid carrying the cue fragment could also replicate in HeLa and NIH3T3 cells.
UR - http://www.scopus.com/inward/record.url?scp=0034157054&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034157054&partnerID=8YFLogxK
U2 - 10.1023/A:1007076511814
DO - 10.1023/A:1007076511814
M3 - Article
C2 - 10939521
AN - SCOPUS:0034157054
VL - 27
SP - 13
EP - 19
JO - Molecular Biology Reports
JF - Molecular Biology Reports
SN - 0301-4851
IS - 1
ER -