Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae

Takahiro Kusakabe, Yasushi Sugimoto, Yoshiko Hirota, Shigenobu Toné, Yutaka Kawaguchi, Katsumi Koga, Takashi Ohyama

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

Two fragments that could function as replicational cue elements were isolated from a genomic DNA digest of Aspergillus oryzae on the basis of abnormal behavior in polyacrylamide gel electrophoresis. The vector used in this study contained a scaffold-associated region of the Drosophila melanogaster ftz gene to provide nuclear retention. Neither fragment contained a yeast ARS consensus sequence or an eukaryotic topoisomerase II binding sequence. One of the fragments showed sequence homology with the mitochondrial replication origin of Candida utilis and a portion of mitochondrial DNA of Aspergillus nidulans. This plasmid carrying the cue fragment could also replicate in HeLa and NIH3T3 cells.

元の言語英語
ページ(範囲)13-19
ページ数7
ジャーナルMolecular Biology Reports
27
発行部数1
DOI
出版物ステータス出版済み - 1 1 2000

Fingerprint

Aspergillus oryzae
Gene Library
Cues
Aspergillus nidulans
Type II DNA Topoisomerase
Replication Origin
Consensus Sequence
Sequence Homology
Drosophila melanogaster
Mitochondrial DNA
HeLa Cells
Candida
Polyacrylamide Gel Electrophoresis
Plasmids
Yeasts
DNA
Genes
Retention (Psychology)

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

これを引用

Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae. / Kusakabe, Takahiro; Sugimoto, Yasushi; Hirota, Yoshiko; Toné, Shigenobu; Kawaguchi, Yutaka; Koga, Katsumi; Ohyama, Takashi.

:: Molecular Biology Reports, 巻 27, 番号 1, 01.01.2000, p. 13-19.

研究成果: ジャーナルへの寄稿記事

Kusakabe, T, Sugimoto, Y, Hirota, Y, Toné, S, Kawaguchi, Y, Koga, K & Ohyama, T 2000, 'Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae', Molecular Biology Reports, 巻. 27, 番号 1, pp. 13-19. https://doi.org/10.1023/A:1007076511814
Kusakabe, Takahiro ; Sugimoto, Yasushi ; Hirota, Yoshiko ; Toné, Shigenobu ; Kawaguchi, Yutaka ; Koga, Katsumi ; Ohyama, Takashi. / Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae. :: Molecular Biology Reports. 2000 ; 巻 27, 番号 1. pp. 13-19.
@article{154acd0cfa6648c2bbab8023b3875f7b,
title = "Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae",
abstract = "Two fragments that could function as replicational cue elements were isolated from a genomic DNA digest of Aspergillus oryzae on the basis of abnormal behavior in polyacrylamide gel electrophoresis. The vector used in this study contained a scaffold-associated region of the Drosophila melanogaster ftz gene to provide nuclear retention. Neither fragment contained a yeast ARS consensus sequence or an eukaryotic topoisomerase II binding sequence. One of the fragments showed sequence homology with the mitochondrial replication origin of Candida utilis and a portion of mitochondrial DNA of Aspergillus nidulans. This plasmid carrying the cue fragment could also replicate in HeLa and NIH3T3 cells.",
author = "Takahiro Kusakabe and Yasushi Sugimoto and Yoshiko Hirota and Shigenobu Ton{\'e} and Yutaka Kawaguchi and Katsumi Koga and Takashi Ohyama",
year = "2000",
month = "1",
day = "1",
doi = "10.1023/A:1007076511814",
language = "English",
volume = "27",
pages = "13--19",
journal = "Molecular Biology Reports",
issn = "0301-4851",
publisher = "Springer Netherlands",
number = "1",

}

TY - JOUR

T1 - Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae

AU - Kusakabe, Takahiro

AU - Sugimoto, Yasushi

AU - Hirota, Yoshiko

AU - Toné, Shigenobu

AU - Kawaguchi, Yutaka

AU - Koga, Katsumi

AU - Ohyama, Takashi

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Two fragments that could function as replicational cue elements were isolated from a genomic DNA digest of Aspergillus oryzae on the basis of abnormal behavior in polyacrylamide gel electrophoresis. The vector used in this study contained a scaffold-associated region of the Drosophila melanogaster ftz gene to provide nuclear retention. Neither fragment contained a yeast ARS consensus sequence or an eukaryotic topoisomerase II binding sequence. One of the fragments showed sequence homology with the mitochondrial replication origin of Candida utilis and a portion of mitochondrial DNA of Aspergillus nidulans. This plasmid carrying the cue fragment could also replicate in HeLa and NIH3T3 cells.

AB - Two fragments that could function as replicational cue elements were isolated from a genomic DNA digest of Aspergillus oryzae on the basis of abnormal behavior in polyacrylamide gel electrophoresis. The vector used in this study contained a scaffold-associated region of the Drosophila melanogaster ftz gene to provide nuclear retention. Neither fragment contained a yeast ARS consensus sequence or an eukaryotic topoisomerase II binding sequence. One of the fragments showed sequence homology with the mitochondrial replication origin of Candida utilis and a portion of mitochondrial DNA of Aspergillus nidulans. This plasmid carrying the cue fragment could also replicate in HeLa and NIH3T3 cells.

UR - http://www.scopus.com/inward/record.url?scp=0034157054&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034157054&partnerID=8YFLogxK

U2 - 10.1023/A:1007076511814

DO - 10.1023/A:1007076511814

M3 - Article

C2 - 10939521

AN - SCOPUS:0034157054

VL - 27

SP - 13

EP - 19

JO - Molecular Biology Reports

JF - Molecular Biology Reports

SN - 0301-4851

IS - 1

ER -