抄録
Two fragments that could function as replicational cue elements were isolated from a genomic DNA digest of Aspergillus oryzae on the basis of abnormal behavior in polyacrylamide gel electrophoresis. The vector used in this study contained a scaffold-associated region of the Drosophila melanogaster ftz gene to provide nuclear retention. Neither fragment contained a yeast ARS consensus sequence or an eukaryotic topoisomerase II binding sequence. One of the fragments showed sequence homology with the mitochondrial replication origin of Candida utilis and a portion of mitochondrial DNA of Aspergillus nidulans. This plasmid carrying the cue fragment could also replicate in HeLa and NIH3T3 cells.
本文言語 | 英語 |
---|---|
ページ(範囲) | 13-19 |
ページ数 | 7 |
ジャーナル | Molecular biology reports |
巻 | 27 |
号 | 1 |
DOI | |
出版ステータス | 出版済み - 2000 |
!!!All Science Journal Classification (ASJC) codes
- 分子生物学
- 遺伝学