Isolation of suppressor mutants of phosphatidylinositol 3-phosphate 5-kinase deficient cells in schizosaccharomyce pombe

Masayuki Onishi, Yoko Nakamura, Takako Koga, Kaoru Takegawa, Yasuhisa Fukui

研究成果: Contribution to journalArticle査読

17 被引用数 (Scopus)

抄録

The ste12+ gene of Schizosaccharomyces pombe codes for a phosphatidylinositol (PI) 3-phosphate 5′-kinase, which is required for efficient mating. Suppressor mutants for sterility of ste12Δ cells were screened for. Most of the mutant genes turned out to be recessive. Six genes were cloned and the open reading frames responsible for the suppressor activity were identified. They included genes coding for proteins with domains homologous to calcium transporters, casein kinase II, UBC13, AMSH, Vps23p, and Vps27p of Saccharomyces cerevisiae. Disruption of these genes resulted in suppression of the defects of the ste12Δ cells, including low mating efficiency and formation of large vacuoles. Since many of these gene products are homologous to the proteins involved in vesicle transport, sterility caused by inactivation of ste12 may be due to a disordered vesicle transport system.

本文言語英語
ページ(範囲)1772-1779
ページ数8
ジャーナルBioscience, Biotechnology and Biochemistry
67
8
DOI
出版ステータス出版済み - 1 1 2003
外部発表はい

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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