TY - JOUR
T1 - Isolation of the First Component of Complement (C1) from Carp Serum
AU - Yano, Tomoki
AU - Matsuyama, Hiroko
AU - Nakao, Miki
PY - 1988
Y1 - 1988
N2 - The first component of complement (C1) was isolated from carp serum by a 6-step purification procedure: 1) affinity chromatography (I) on Blue Cellulofine, 2) QAE-Sephadex A-50 chromatography, 3) gel filtration on Sepharose CL-6B, 4) Blue Cellulofine chromatography (II), 5) DEAE-Toyopearl 650 m chromatography (I), and 6) DEAE-Toyopearl 650M chromatography (II). The final recovery of C1 hemolytic activity was 18%, representing a 788-fold purification. The purity of the isolated carp C1 was established by gel filtration on Sepharose CL-6B and by immunoelectrophoresis against anti-whole carp serum (rabbit). The molecular weight of carp C1 was estimated to be 1,020,000. The hemolytic activity of carp C1 was inhibited by EDTA treatment, but this inhibition was overcome by dialysis against a Ca2+-containing buffer. The hemolytic activity of carp C1 was destroyed by heating (50°C, 10 min) or incubation with carrageenan, but was retained when incubated with ammonia, hydrazine or zymosan. This indicates that carp C1 resembles mammalian C1 in chemical properties.
AB - The first component of complement (C1) was isolated from carp serum by a 6-step purification procedure: 1) affinity chromatography (I) on Blue Cellulofine, 2) QAE-Sephadex A-50 chromatography, 3) gel filtration on Sepharose CL-6B, 4) Blue Cellulofine chromatography (II), 5) DEAE-Toyopearl 650 m chromatography (I), and 6) DEAE-Toyopearl 650M chromatography (II). The final recovery of C1 hemolytic activity was 18%, representing a 788-fold purification. The purity of the isolated carp C1 was established by gel filtration on Sepharose CL-6B and by immunoelectrophoresis against anti-whole carp serum (rabbit). The molecular weight of carp C1 was estimated to be 1,020,000. The hemolytic activity of carp C1 was inhibited by EDTA treatment, but this inhibition was overcome by dialysis against a Ca2+-containing buffer. The hemolytic activity of carp C1 was destroyed by heating (50°C, 10 min) or incubation with carrageenan, but was retained when incubated with ammonia, hydrazine or zymosan. This indicates that carp C1 resembles mammalian C1 in chemical properties.
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U2 - 10.2331/suisan.54.851
DO - 10.2331/suisan.54.851
M3 - Article
AN - SCOPUS:85008126144
VL - 54
SP - 851
EP - 859
JO - Nippon Suisan Gakkaishi
JF - Nippon Suisan Gakkaishi
SN - 0021-5392
IS - 5
ER -