Isolation of the Second Component of Complement (C2) from Carp Serum

Takeshi Uemura, Miki Nakao, Tomoki Yano

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

The second component (C2) of carp complement was isolated from carp serum by a six-step purification procedure: 1) affinity chromatography on Blue-Cellulofine, 2) affinity chromatography on heparin-agarose, 3) gel filtration on Sephadex G-200, 4) TSKgel DEAE-5PW chromatography (HPLC), 5) TSKgel Heparin-5PW chromatography (HPLC), and 6) TSKgel G3O0OSWXL chromatography (HPLC). Hemolytic activity of carp C2 in column eluates was measured using EAC14 (sheep erythrocytes sensitized with carp antibody, Cl and C4) and heat-inactivated carp serum (deficient in Cl and C2) as reagents for detecting C2. About 0.4 mg of carp C2 was obtained from 100 ml of serum. The final recovery was 9.4%, representing a 799-fold purification. Carp C2 was composed of a single polypeptide chain with molecular weight of 108,000 and was susceptible to heat-inactivation at 45°C. These physicochemical properties of carp C2 closely resemble those reported for mammalian C2.

元の言語英語
ページ(範囲)727-733
ページ数7
ジャーナルNIPPON SUISAN GAKKAISHI
58
発行部数4
DOI
出版物ステータス出版済み - 1 1 1992

Fingerprint

carp
chromatography
serum
complement
purification
high performance liquid chromatography
heparin
affinity chromatography
physicochemical property
sheep
antibody
gel
heat inactivation
fold
agarose
polypeptides
physicochemical properties
erythrocytes
gels
molecular weight

All Science Journal Classification (ASJC) codes

  • Aquatic Science

これを引用

Isolation of the Second Component of Complement (C2) from Carp Serum. / Uemura, Takeshi; Nakao, Miki; Yano, Tomoki.

:: NIPPON SUISAN GAKKAISHI, 巻 58, 番号 4, 01.01.1992, p. 727-733.

研究成果: ジャーナルへの寄稿記事

Uemura, Takeshi ; Nakao, Miki ; Yano, Tomoki. / Isolation of the Second Component of Complement (C2) from Carp Serum. :: NIPPON SUISAN GAKKAISHI. 1992 ; 巻 58, 番号 4. pp. 727-733.
@article{32305e3ab2bb40f882755bf8034889f7,
title = "Isolation of the Second Component of Complement (C2) from Carp Serum",
abstract = "The second component (C2) of carp complement was isolated from carp serum by a six-step purification procedure: 1) affinity chromatography on Blue-Cellulofine, 2) affinity chromatography on heparin-agarose, 3) gel filtration on Sephadex G-200, 4) TSKgel DEAE-5PW chromatography (HPLC), 5) TSKgel Heparin-5PW chromatography (HPLC), and 6) TSKgel G3O0OSWXL chromatography (HPLC). Hemolytic activity of carp C2 in column eluates was measured using EAC14 (sheep erythrocytes sensitized with carp antibody, Cl and C4) and heat-inactivated carp serum (deficient in Cl and C2) as reagents for detecting C2. About 0.4 mg of carp C2 was obtained from 100 ml of serum. The final recovery was 9.4{\%}, representing a 799-fold purification. Carp C2 was composed of a single polypeptide chain with molecular weight of 108,000 and was susceptible to heat-inactivation at 45°C. These physicochemical properties of carp C2 closely resemble those reported for mammalian C2.",
author = "Takeshi Uemura and Miki Nakao and Tomoki Yano",
year = "1992",
month = "1",
day = "1",
doi = "10.2331/suisan.58.727",
language = "English",
volume = "58",
pages = "727--733",
journal = "Nippon Suisan Gakkaishi",
issn = "0021-5392",
publisher = "Nihon Suisan Gakkai",
number = "4",

}

TY - JOUR

T1 - Isolation of the Second Component of Complement (C2) from Carp Serum

AU - Uemura, Takeshi

AU - Nakao, Miki

AU - Yano, Tomoki

PY - 1992/1/1

Y1 - 1992/1/1

N2 - The second component (C2) of carp complement was isolated from carp serum by a six-step purification procedure: 1) affinity chromatography on Blue-Cellulofine, 2) affinity chromatography on heparin-agarose, 3) gel filtration on Sephadex G-200, 4) TSKgel DEAE-5PW chromatography (HPLC), 5) TSKgel Heparin-5PW chromatography (HPLC), and 6) TSKgel G3O0OSWXL chromatography (HPLC). Hemolytic activity of carp C2 in column eluates was measured using EAC14 (sheep erythrocytes sensitized with carp antibody, Cl and C4) and heat-inactivated carp serum (deficient in Cl and C2) as reagents for detecting C2. About 0.4 mg of carp C2 was obtained from 100 ml of serum. The final recovery was 9.4%, representing a 799-fold purification. Carp C2 was composed of a single polypeptide chain with molecular weight of 108,000 and was susceptible to heat-inactivation at 45°C. These physicochemical properties of carp C2 closely resemble those reported for mammalian C2.

AB - The second component (C2) of carp complement was isolated from carp serum by a six-step purification procedure: 1) affinity chromatography on Blue-Cellulofine, 2) affinity chromatography on heparin-agarose, 3) gel filtration on Sephadex G-200, 4) TSKgel DEAE-5PW chromatography (HPLC), 5) TSKgel Heparin-5PW chromatography (HPLC), and 6) TSKgel G3O0OSWXL chromatography (HPLC). Hemolytic activity of carp C2 in column eluates was measured using EAC14 (sheep erythrocytes sensitized with carp antibody, Cl and C4) and heat-inactivated carp serum (deficient in Cl and C2) as reagents for detecting C2. About 0.4 mg of carp C2 was obtained from 100 ml of serum. The final recovery was 9.4%, representing a 799-fold purification. Carp C2 was composed of a single polypeptide chain with molecular weight of 108,000 and was susceptible to heat-inactivation at 45°C. These physicochemical properties of carp C2 closely resemble those reported for mammalian C2.

UR - http://www.scopus.com/inward/record.url?scp=85008106050&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85008106050&partnerID=8YFLogxK

U2 - 10.2331/suisan.58.727

DO - 10.2331/suisan.58.727

M3 - Article

AN - SCOPUS:85008106050

VL - 58

SP - 727

EP - 733

JO - Nippon Suisan Gakkaishi

JF - Nippon Suisan Gakkaishi

SN - 0021-5392

IS - 4

ER -