Kid depletion in mouse oocytes associated with multinucleated blastomere formation and inferior embryo development

Akiyoshi Egashira, Nobuhiko Yamauchi, Md Rashedul Islam, Kazuki Yamagami, Asami Tanaka, Hikaru Suyama, El Sharawy Mohamed El-Sayed, Shoji Tabata, Takashi Kuramoto

研究成果: Contribution to journalArticle査読

抄録

This study investigated the knockdown (KD) of Kid on maturation developmental competence and multinucleation of mouse germinal vesicle (GV) oocytes after parthenogenetic activation. Data revealed that Kid messenger RNA (mRNA) was expressed in GV and MII stage oocyte and 1- and 2-cell embryos. Additionally, Kid mRNA expression in the Kid KD group decreased by nearly 46% compared to the control small interfering RNA (siRNA) groups. The rate of multinucleated embryos in the Kid KD group (52.4%) was significantly higher (P < 0.05) than the control siRNA group (4.7%). Finally, the developmental rates were significantly lower in the Kid siRNA group at > 4-cell stage (28.6% vs. 53.5%) and the blastocyst stage (2.4% vs. 23.3%) compared to the control siRNA groups. Suppression of Kid using siRNA caused multinucleation in early embryos with high frequency and it may increase 2- to 4-cell arrested embryos and reduce the developmental competence to blastocyst.

本文言語英語
ページ(範囲)1048-1054
ページ数7
ジャーナルAnimal science journal = Nihon chikusan Gakkaiho
87
8
DOI
出版ステータス出版済み - 8 1 2016

All Science Journal Classification (ASJC) codes

  • 農業および生物科学(全般)

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