Kinesin heads fused to hinge-free myosin tails drive efficient motility

Verena Eickel, Douglas Robert Drummond, Nick Carter, Andrew Lockhart, John Kendrick Jones, Robert Cross

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

The rat kinesin motor domain was fused at residues 433, 411, 376 or 367, respectively, to the C-terminal 1185, 1187, 1197 or 1185 residues of the brush border myosin tail. In motility assays, K433myt and K411myt, which preserve the head-proximal kinesin hinge, and K367myt, which deletes it, drove rapid microtubule sliding (∼0.6 μms-1) that was optimal when the head-pairs were spaced apart by adding 1:1 headless myosin tails. K376myt, which partially deletes the head-proximal hinge, showed poor motility in sliding assays but wild type processivity, velocity and stall force in single molecule optical trapping. Accordingly, the head-proximal kinesin hinge is functionally dispensable.

元の言語英語
ページ(範囲)54-58
ページ数5
ジャーナルFEBS Letters
569
発行部数1-3
DOI
出版物ステータス出版済み - 7 2 2004

Fingerprint

Kinesin
Hinges
Myosins
Tail
Head
Assays
Optical Tweezers
Brushes
Rats
Microvilli
Microtubules
Molecules

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

これを引用

Kinesin heads fused to hinge-free myosin tails drive efficient motility. / Eickel, Verena; Drummond, Douglas Robert; Carter, Nick; Lockhart, Andrew; Jones, John Kendrick; Cross, Robert.

:: FEBS Letters, 巻 569, 番号 1-3, 02.07.2004, p. 54-58.

研究成果: ジャーナルへの寄稿記事

Eickel, V, Drummond, DR, Carter, N, Lockhart, A, Jones, JK & Cross, R 2004, 'Kinesin heads fused to hinge-free myosin tails drive efficient motility', FEBS Letters, 巻. 569, 番号 1-3, pp. 54-58. https://doi.org/10.1016/j.febslet.2004.05.050
Eickel, Verena ; Drummond, Douglas Robert ; Carter, Nick ; Lockhart, Andrew ; Jones, John Kendrick ; Cross, Robert. / Kinesin heads fused to hinge-free myosin tails drive efficient motility. :: FEBS Letters. 2004 ; 巻 569, 番号 1-3. pp. 54-58.
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