TY - JOUR
T1 - Kinetic characteristics of biochemical cyclic reaction systems
T2 - Amplification of substrate cycle system
AU - Okamoto, Masahiro
AU - Hayashi, Katsuya
PY - 1985/2
Y1 - 1985/2
N2 - The amplification of a substrate cycle system with reversible closed reaction of two substrates was represented by mathematical equations. The results are summarized as follows: the amplification was affected especially by the affinity of enzyme and substrate, by the rate constant in rate‐limiting reaction step, and by the saturation degree of enzyme by substrate. These amplifications were not simply determined by the values of Km and Vmax, because each rate parameter in the system can affect the degree of amplification independently. The conclusion is that the “apparent” equilibrium constant of this system cannot be uniquely estimated from only data of Km and Vmax even if the reaction occurs in a closed system.
AB - The amplification of a substrate cycle system with reversible closed reaction of two substrates was represented by mathematical equations. The results are summarized as follows: the amplification was affected especially by the affinity of enzyme and substrate, by the rate constant in rate‐limiting reaction step, and by the saturation degree of enzyme by substrate. These amplifications were not simply determined by the values of Km and Vmax, because each rate parameter in the system can affect the degree of amplification independently. The conclusion is that the “apparent” equilibrium constant of this system cannot be uniquely estimated from only data of Km and Vmax even if the reaction occurs in a closed system.
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U2 - 10.1002/bit.260270205
DO - 10.1002/bit.260270205
M3 - Article
AN - SCOPUS:0022012737
VL - 27
SP - 132
EP - 136
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
SN - 0006-3592
IS - 2
ER -