Kinetic characteristics of biochemical cyclic reaction systems: Amplification of substrate cycle system

Masahiro Okamoto, Katsuya Hayashi

研究成果: Contribution to journalArticle査読

3 被引用数 (Scopus)

抄録

The amplification of a substrate cycle system with reversible closed reaction of two substrates was represented by mathematical equations. The results are summarized as follows: the amplification was affected especially by the affinity of enzyme and substrate, by the rate constant in rate‐limiting reaction step, and by the saturation degree of enzyme by substrate. These amplifications were not simply determined by the values of Km and Vmax, because each rate parameter in the system can affect the degree of amplification independently. The conclusion is that the “apparent” equilibrium constant of this system cannot be uniquely estimated from only data of Km and Vmax even if the reaction occurs in a closed system.

本文言語英語
ページ(範囲)132-136
ページ数5
ジャーナルBiotechnology and Bioengineering
27
2
DOI
出版ステータス出版済み - 2 1985

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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