Kinetic modeling and sensitivity analysis of acetone-butanol-ethanol production

Hideaki Shinto, Yukihiro Tashiro, Mayu Yamashita, Genta Kobayashi, Tatsuya Sekiguchi, Taizo Hanai, Yuki Kuriya, Masahiro Okamoto, Kenji Sonomoto

研究成果: ジャーナルへの寄稿記事

78 引用 (Scopus)

抄録

A kinetic simulation model of metabolic pathways that describes the dynamic behaviors of metabolites in acetone-butanol-ethanol (ABE) production by Clostridium saccharoperbutylacetonicum N1-4 was proposed using a novel simulator WinBEST-KIT. This model was validated by comparing with experimental time-course data of metabolites in batch cultures over a wide range of initial glucose concentrations (36.1-295 mM). By introducing substrate inhibition, product inhibition of butanol, activation of butyrate and considering the cessation of metabolic reactions in the case of insufficiency of energy after glucose exhaustion, the revised model showed 0.901 of squared correlation coefficient (r2) between experimental time-course of metabolites and calculated ones. Thus, the final revised model is assumed to be one of the best candidates for kinetic simulation describing dynamic behavior of metabolites in ABE production. Sensitivity analysis revealed that 5% increase in reaction of reverse pathway of butyrate production (R17) and 5% decrease in reaction of CoA transferase for butyrate (R15) highly contribute to high production of butanol. These system analyses should be effective in the elucidation which pathway is metabolic bottleneck for high production of butanol.

元の言語英語
ページ(範囲)45-56
ページ数12
ジャーナルJournal of Biotechnology
131
発行部数1
DOI
出版物ステータス出版済み - 8 1 2007

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Butanols
Acetone
Butenes
Sensitivity analysis
Metabolites
Ethanol
Butyrates
Kinetics
Metabolic Networks and Pathways
Glucose
Coenzyme A-Transferases
Clostridium
Batch Cell Culture Techniques
Systems Analysis
Simulators
Chemical activation
Substrates

All Science Journal Classification (ASJC) codes

  • Biotechnology

これを引用

Kinetic modeling and sensitivity analysis of acetone-butanol-ethanol production. / Shinto, Hideaki; Tashiro, Yukihiro; Yamashita, Mayu; Kobayashi, Genta; Sekiguchi, Tatsuya; Hanai, Taizo; Kuriya, Yuki; Okamoto, Masahiro; Sonomoto, Kenji.

:: Journal of Biotechnology, 巻 131, 番号 1, 01.08.2007, p. 45-56.

研究成果: ジャーナルへの寄稿記事

Shinto, H, Tashiro, Y, Yamashita, M, Kobayashi, G, Sekiguchi, T, Hanai, T, Kuriya, Y, Okamoto, M & Sonomoto, K 2007, 'Kinetic modeling and sensitivity analysis of acetone-butanol-ethanol production', Journal of Biotechnology, 巻. 131, 番号 1, pp. 45-56. https://doi.org/10.1016/j.jbiotec.2007.05.005
Shinto, Hideaki ; Tashiro, Yukihiro ; Yamashita, Mayu ; Kobayashi, Genta ; Sekiguchi, Tatsuya ; Hanai, Taizo ; Kuriya, Yuki ; Okamoto, Masahiro ; Sonomoto, Kenji. / Kinetic modeling and sensitivity analysis of acetone-butanol-ethanol production. :: Journal of Biotechnology. 2007 ; 巻 131, 番号 1. pp. 45-56.
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abstract = "A kinetic simulation model of metabolic pathways that describes the dynamic behaviors of metabolites in acetone-butanol-ethanol (ABE) production by Clostridium saccharoperbutylacetonicum N1-4 was proposed using a novel simulator WinBEST-KIT. This model was validated by comparing with experimental time-course data of metabolites in batch cultures over a wide range of initial glucose concentrations (36.1-295 mM). By introducing substrate inhibition, product inhibition of butanol, activation of butyrate and considering the cessation of metabolic reactions in the case of insufficiency of energy after glucose exhaustion, the revised model showed 0.901 of squared correlation coefficient (r2) between experimental time-course of metabolites and calculated ones. Thus, the final revised model is assumed to be one of the best candidates for kinetic simulation describing dynamic behavior of metabolites in ABE production. Sensitivity analysis revealed that 5{\%} increase in reaction of reverse pathway of butyrate production (R17) and 5{\%} decrease in reaction of CoA transferase for butyrate (R15) highly contribute to high production of butanol. These system analyses should be effective in the elucidation which pathway is metabolic bottleneck for high production of butanol.",
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AU - Hanai, Taizo

AU - Kuriya, Yuki

AU - Okamoto, Masahiro

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